Construct: ORF TRCN0000466768
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016181.1_s317c1
- Derived from:
- ccsbBroadEn_05356
- DNA Barcode:
- GTGTGCCCTGCACCCCACTCTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAS2L3 (283431)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466768
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283431 | GAS2L3 | growth arrest specific 2 li... | NM_001303130.2 | 99.9% | 100% | 862T>C |
| 2 | human | 283431 | GAS2L3 | growth arrest specific 2 li... | NM_001363672.2 | 99.9% | 100% | 862T>C |
| 3 | human | 283431 | GAS2L3 | growth arrest specific 2 li... | NM_174942.3 | 99.9% | 100% | 862T>C |
| 4 | human | 283431 | GAS2L3 | growth arrest specific 2 li... | XM_005268804.3 | 99.9% | 100% | 862T>C |
| 5 | human | 283431 | GAS2L3 | growth arrest specific 2 li... | XM_011538220.2 | 99.9% | 100% | 862T>C |
| 6 | human | 283431 | GAS2L3 | growth arrest specific 2 li... | XM_011538221.2 | 99.9% | 100% | 862T>C |
| 7 | human | 283431 | GAS2L3 | growth arrest specific 2 li... | XM_017019206.1 | 99.9% | 100% | 862T>C |
| 8 | human | 283431 | GAS2L3 | growth arrest specific 2 li... | NM_001303131.1 | 84.9% | 85% | 0_1ins312;550T>C |
| 9 | mouse | 237436 | Gas2l3 | growth arrest-specific 2 li... | NM_001284344.1 | 45.2% | 47.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2148
- ORF length:
- 2082
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gcctgcaatt caagtatggt ttggagaaga tctgcctcta agtcctcgga 121 gtcctctgac tcccagacac ggaccaggat tggctaatgt ttgtcagtac gatgagtgga 181 tagctgtgag gcatgaagcc actttgttgc ccatgcaaga agatctgtca atctggttat 241 ctggtttatt aggtattaaa gttaaggcag aaaaattatt ggaagaactt gataatggag 301 tactattatg tcaactgatt gatgttcttc aaaacatggt gaaaacatgc aactctgaag 361 aatcagggaa ttttccaatg agaaaagtgc cctgtaagaa agatgctgca tcaggttcat 421 tctttgctcg ggacaatacc gcaaacttcc ttcactggtg tagggacatt ggggttgatg 481 aaacttacct ctttgaatct gaaggtttag ttttgcacaa agatccaaga caggtgtatc 541 tttgtcttct tgaaattggt cgaattgtgt caagatacgg ggttgagcca ccagtgttag 601 taaaacttga gaaagaaatt gagttagaag agactttgct taatacttct gggcctgaag 661 attccatcag cattccaaaa tcatgctgtc ggcatgaaga gctacatgaa gctgttaaac 721 atattgctga ggaccctcct tgtagttgtt ctcatcgatt ttctattgag tatttatctg 781 aaggacggta ccgactaggg gataaaatac tctttataag aatgcttcat ggaaaacatg 841 tcatggttcg cgttggtgga ggctgggata ctcttcaagg atttttgctt aaatatgacc 901 cctgtcgaat attacagttt gccacactag aacaaaaaat tttagcattt caaaaaggag 961 tttctaatga aagtgtacct gattcgcctg ccagaacacc tcagcctcct gaaatgaatc 1021 ctttgtcagc agttaacatg tttcagaaac aaaattcaaa acccagcgtg ccagttagta 1081 ttccaaaaag caaagaaaaa cagggacgtc caccaggtgc attggtgcca gcatcttcac 1141 tgaaaggagg taatctgggc tctatgtcag tccgttctaa attgccaaat tctccagcag 1201 catcttctca tcccaagctc aagtcttcaa aaggcataac gaagaaaccg caggctcctt 1261 caaacaatgc atcatcttca cttgcttcat taaatccagt aggtaaaaac acttcttcac 1321 cagctttacc aagaactgca ccttgtatat ctgagtcacc gagaaaatgt atttcatccc 1381 ccaatacccc caaggccaag gttattccag cccagaattc agcagatctg cccgagtcca 1441 cacttttgcc aaataagtgt tcaggaaaaa ctcaacctaa gtatttgaaa cataatcata 1501 tttcttccag agataatgca gtatctcact tagctgcaca ttcaaattca tcctcaaaat 1561 gtcccaagct gcctaaagca aatatacctg taagacctaa accttctttc cagtcctctg 1621 caaaaatgac aaaaaccagt tccaaaacca tagccacggg tctaggaaca cagtctcaac 1681 catccgatgg agccccacaa gcaaagccag tcccagcaca gaaacttaaa tcggccttga 1741 atttaaatca gccagtttct gtgtccTCAG TTTCTCCTGT AAAAGCCACA CAGAAATCAA 1801 AAGATAAGAA TATAGTTTCA GCTACCAAAA AGCAGCCTCA GAATAAAAGT GCATTTCAGA 1861 AGACAGGACC CAGCTCCTTG AAGTCTCCTG GCCGTACCCC ACTGTCCATC GTGAGCCTAC 1921 CCCAGTCTTC TACCAAAACA CAAACTGCAC CGAAGTCAGC ACAGACTGTC GCTAAGAGCC 1981 AGCATTCAAC TAAAGGGCCT CCCAGAAGTG GCAAAACCCC AGCTTCAATC AGGAAACCAC 2041 CCTCATCTGT TAAGGATGCA GATAGTGGAG ATAAAAAACC TACTGCAAAG AAAAAGGAAG 2101 ATGATGACCA TTATTTTGTC ATGACTGGAA GTAAGAAACC TAGAAAATAC CCAACTTTCT 2161 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2221 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2281 GTGGAAAGGA CGAGTGTGCC CTGCACCCCA CTCTTGCACG CGTTAAGTCg acaatcaacc 2341 tctggattac aaaatttgtg aaagatt