Construct: ORF TRCN0000466771
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013061.1_s317c1
- Derived from:
- ccsbBroadEn_09630
- DNA Barcode:
- AACGTTTTGAGAATACGGCTCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C16orf71 (146562)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466771
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146562 | C16orf71 | chromosome 16 open reading ... | NM_139170.3 | 99.4% | 99% | (many diffs) |
| 2 | human | 146562 | C16orf71 | chromosome 16 open reading ... | XM_017022976.1 | 99.4% | 99% | (many diffs) |
| 3 | human | 146562 | C16orf71 | chromosome 16 open reading ... | XM_017022975.1 | 95.2% | 94.8% | (many diffs) |
| 4 | human | 146562 | C16orf71 | chromosome 16 open reading ... | XM_005255144.3 | 73% | 72.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1623
- ORF length:
- 1557
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc atccaacgat aaaggcatgg caccctcgct gggctctccc tgggcctccc 121 ggatggggcc ctgggatgcc atcctcaagg ctgtcaaaga ccagctcccg tctctggact 181 cagactcccc tttgtcggac tatggggaag aggagctgtt catcttccag cgaaaccaaa 241 cctccctgat tccagacctg tcggaggagc tggctgaaga tcctgccgat ggcgacaagt 301 ccagggcctg ggtcgctgca gctgaagagt cccttcccga gccagttctg gtgcctgcag 361 aattggccac agaacctggg tgcagacaga acacaaggac aaaggatgca tcctctcagg 421 aaggaagaga ccctggcagg ccttttgaaa gctctggtga agtcagcgct cttcttggga 481 tggccgagga gccccccagg tggctggaag gcgaccttgg aagcctgtct ttcaacacca 541 aaggatccca gggtcctccc tgggacccac aggccgaagc cactctctcc tgccatgaag 601 gagacccaaa ggcagagccc ctcagcactg cctcacaaga atctgtgaac cgccgggccc 661 tccgacagga gagaaggaag atgatagaga cggacatcct ccagaaagtc acccgggatg 721 cctgcggccc gaccagcagt gacaaaggtg gggtgaagga ggcgccctgc cacgctgcgg 781 agtcagctcc cagatccaaa atgcccctcg tggagcctcc ggagggacca ccagtgctct 841 cgctccagca acttgaagcg tgggatttgg atgacatcct tcagagtctg gcgggacaag 901 aagacaacca gggaaatcgt gcacctggaa ctgtgtggtg ggcagctgac caccgccaag 961 ttcaagactg catggtgccg agcgcccaca acaggctcat ggaacagctg gccctcctgt 1021 gcaccacgca gtccaaggcc tctgcttgtg cccggaaggt gcctgccgac actccccagg 1081 acaccaaaga ggcagattca ggaagcagat gtgcctcaag gaagcggggc tcccaggctg 1141 ggccaggccc gcagctggcc cagggcatga ggcttaacgc agagtccccc accatcttta 1201 ttgaccTGCG GCAGATGGAG CTACCAGACC ACCTGTCCCC AGAAAGCTCC AGCCACAGCT 1261 CCTCTGACAG TGAGGAGGAG GAGGAAGAGA TGGCAGCTCT GGGAGACGCA GAGGGGGCAT 1321 CTCCTTCCTC CCTGGGGCTA CGGACCTGTA CCGGGAAAAG CCAGCTTCTC CAGCAGCTCA 1381 GGGCCTTTCA GAAGGGGACA GCCCAGCCCG AGCTGCCTGC CAGCAAGGGG CCCGCGGGTG 1441 GGAGGGCTCA GGCCCTTGAA GACACAGCTG GATCACGAAC TGGGAGGAAG CAACACATGA 1501 AGCTCTGTGC CAAGGGGCAG AGCGCCCAGG CTCGACTCCC AAGAGGCAGG CCCAGAGCCC 1561 TGGGGGATGT TCCTGAGCCA GGGGCAGCCA GGGAGGCCCT GATGCCTCCT CTGGAGCAAC 1621 TATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1681 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1741 GGCTTTATAT ATCTTGTGGA AAGGACGAAA CGTTTTGAGA ATACGGCTCA ACACGCGTTA 1801 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt