Construct: ORF TRCN0000466782

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012940.1_s317c1
Derived from:
ccsbBroadEn_03884
DNA Barcode:
ACTGGGTCACAGTAGTGATTACCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TGIF2 (60436)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466782
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 60436 TGIF2 TGFB induced factor homeobox 2 NM_001199513.1 100% 100%
2 human 60436 TGIF2 TGFB induced factor homeobox 2 NM_001199514.2 100% 100%
3 human 60436 TGIF2 TGFB induced factor homeobox 2 NM_001199515.1 100% 100%
4 human 60436 TGIF2 TGFB induced factor homeobox 2 NM_021809.7 100% 100%
5 mouse 228839 Tgif2 TGFB-induced factor homeobox 2 NM_173396.3 88.4% 93.6% (many diffs)
6 mouse 228839 Tgif2 TGFB-induced factor homeobox 2 XM_006499351.3 88.4% 93.6% (many diffs)
7 mouse 228839 Tgif2 TGFB-induced factor homeobox 2 XM_006499352.3 88.4% 93.6% (many diffs)
8 mouse 228839 Tgif2 TGFB-induced factor homeobox 2 XM_006499353.3 88.4% 93.6% (many diffs)
9 mouse 228839 Tgif2 TGFB-induced factor homeobox 2 XM_006499354.3 88.4% 93.6% (many diffs)
10 mouse 228839 Tgif2 TGFB-induced factor homeobox 2 NM_001291124.1 73.8% 78% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
777
ORF length:
711
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc ggacagtgat ctaggtgagg acgaaggcct cctctccctg gcgggcaaaa
121ggaagcgcag ggggaacctg cccaaggagt cggtgaagat cctccgggac tggctgtact
181tgcaccgcta caacgcctac ccctcagagc aggagaagct gagcctttct ggacagacca
241acctgtcagt gctgcaaata tgtaactggt tcatcaatgc ccggcggcgg cttctcccag
301acatgcttcg gaaggatggc aaagacccta atcagtttac catttcccgc cgcgggggta
361aggcctcaga tgtggccctc ccccgtggca gcagcccctc agtgctggct gtgtctgtcc
421cagcccccac caatgtgctc tccctgtctg tgtgctccat gccgcttcac tcaggccagg
481gggaaaagcc agcagcccct ttcccacgtg gggagctgga gtctcccaag cccctggtga
541cccctggtag cacacttact cTGCTGACCA GGGCTGAGGC TGGAAGCCCC ACAGGTGGAC
601TCTTCAACAC GCCACCACCC ACACCCCCAG AGCAGGACAA AGAGGACTTC AGCAGCTTCC
661AGCTGCTGGT GGAGGTGGCG CTACAGAGGG CTGCTGAGAT GGAGCTTCAG AAGCAGCAGG
721ACCCATCACT CCCATTACTG CACACTCCCA TCCCTTTAGT CTCTGAAAAT CCCCAGTACC
781CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
841TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
901ATATATCTTG TGGAAAGGAC GAACTGGGTC ACAGTAGTGA TTACCCACGC GTTAAGTCga
961caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)