Construct: ORF TRCN0000466790

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001972.1_s317c1
Derived from:
ccsbBroadEn_10409
DNA Barcode:
TCCCTAGAATGCAATACTATTATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LSP1P3 (729862)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466790
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 729862 LSP1P3 LSP1 pseudogene 3 NR_033961.1 64.8% 1_99del;388_444del
2 human 6801 STRN striatin XM_005264519.5 11% 9.8% (many diffs)
3 human 6801 STRN striatin NM_003162.4 10.5% 9.4% (many diffs)
4 human 6801 STRN striatin XM_011533073.2 10.1% 9% (many diffs)
5 mouse 268980 Strn striatin, calmodulin bindin... NM_011500.2 9.8% 9.4% (many diffs)
6 mouse 268980 Strn striatin, calmodulin bindin... XM_006524357.2 9.4% 9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
93
ORF end:
381
ORF length:
288
Sequence:
1TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT
61GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGAGCAA CAAAAGCTTC TGGGAGCAGA
121AGGGAGGCTC CAAGACCTCA TCAACAATTA CGGCCCAGAT TGCCTTCCTA CAGGGAGAAA
181GGAAGGGCCA AGAAAATTTG AAGAAGGATC TCGTGAGGAT GATCAGAATG TTGGAGTATG
241CTCTTAAACA GAAAAGAGCC AAATACCACA AGTTGAAATA TGGGACAGAA TTGAATCAGG
301GAGCTATGAA GCCTCCAAGC TATGATTCTG ATGAAGGTAA TGAAACAGAA GTGCAGCCAC
361AACAAAACAG CCAGTTAATG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
421CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
481AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCCC TAGAATGCAA
541TACTATTATC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)