Construct: ORF TRCN0000466802
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006882.1_s317c1
- Derived from:
- ccsbBroadEn_12968
- DNA Barcode:
- ATGCCCGGGGAGTCGTACGTAAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LONRF1 (91694)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466802
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91694 | LONRF1 | LON peptidase N-terminal do... | XM_011544694.3 | 76.7% | 76.7% | 1_378del |
| 2 | human | 91694 | LONRF1 | LON peptidase N-terminal do... | NM_152271.5 | 53.8% | 53.8% | 1_1071del |
| 3 | human | 91694 | LONRF1 | LON peptidase N-terminal do... | NM_001329976.2 | 52.3% | 52.3% | 1_1071del;1352_1353ins33 |
| 4 | human | 91694 | LONRF1 | LON peptidase N-terminal do... | XR_002956651.1 | 33.9% | 1_1137del;1180_1268del;2475_3673del | |
| 5 | human | 91694 | LONRF1 | LON peptidase N-terminal do... | XR_002956652.1 | 33% | (many diffs) | |
| 6 | human | 91694 | LONRF1 | LON peptidase N-terminal do... | NR_138255.2 | 31.1% | 1_1177del;1672_1673ins122;2304_3496del | |
| 7 | human | 91694 | LONRF1 | LON peptidase N-terminal do... | NR_138256.2 | 30.2% | (many diffs) | |
| 8 | mouse | 244421 | Lonrf1 | LON peptidase N-terminal do... | NM_001081150.1 | 42.4% | 44.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1317
- ORF length:
- 1248
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaagagtct cagtctctca atgaacctag cccaaagcag agtgaagaaa 121 taccagaggt cacttcagag cctgtcaaag gaagcttaaa ccgtgctcag tcagcacagt 181 ctataaattc aacagaaatg cctgccagag aggactgttt aaaaagagtg tcctcagaac 241 ctgttctgtc agttcaagaa aaaggtgttc tgctgaaaag aaagttgtct cttttagaac 301 aggatgtgat tgtaaatgaa gatggaagaa ataagctgaa aaaacaagga gaaactccca 361 atgaagtctg tatgttttcc ttagcttatg gtgatattcc agaagaatta atcgatgtct 421 cagatttcga gtgttctctc tgcatgaggt tgttttttga gccagtaaca accccttgcg 481 gacattcgtt ctgtaagaat tgtcttgagc gttgtttaga tcatgcacca tattgtcctc 541 tttgcaaaga aagcttaaaa gagtatctag cagataggag gtactgtgtc acacagctgt 601 tggaagaatt aatagtgaag tatctgcctg atgaactgtc tgagagaaaa aaaatatatg 661 atgaagaaac tgctgaactc tcacacttga ccaagaatgt tccaatattt gtttgcacta 721 tggcctaccc cactgtgcct tgccctctcc atgtatttga gccaagatac agattgatga 781 ttcgaagaag tatacagact ggaaccaaac agtttggcat gtgtgtcagt gatacacaaa 841 atagttttgc agattatggt tgtatgttac aaattagaaa cgtgcatttc ttaccggacg 901 gaaggtctgt ggttgataca gttggaggaa agcggtttag ggttttaaaa agaggaatga 961 aagatggata ttgcactgcc gacattgaat atctggaaga tgttaaggtt gagaatgaag 1021 atgagattaa gaatcTCAGA GAGCTTCATG ATTTGGTTTA CTCTCAAGCC TGCAGCTGGT 1081 TTCAGAATTT AAGAGACAGA TTTCGAAGCC AAATTCTTCA GCATTTCGGA TCAATGCCCG 1141 AGAGGGAGGA AAACCTTCAG GCAGCCCCTA ATGGACCTGC ATGGTGTTGG TGGCTTCTTG 1201 CAGTTCTCCC TGTAGACCCA CGATACCAGC TGTCGGTTTT GTCAATGAAG TCTTTGAAAG 1261 AACGGTTGAC CAAGATACAG CATATACTGA CCTATTTTTC TAGAGACCAA TCTAAGTTGC 1321 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GAATGCCCGG GGAGTCGTAC GTAAGGACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt