Construct: ORF TRCN0000466805
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010349.1_s317c1
- Derived from:
- ccsbBroadEn_06418
- DNA Barcode:
- CCTCCAACTGCAGAATGATGGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HTR2B (3357)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466805
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3357 | HTR2B | 5-hydroxytryptamine recepto... | NM_000867.5 | 99.9% | 99.7% | 1430A>G |
| 2 | human | 3357 | HTR2B | 5-hydroxytryptamine recepto... | XM_006712482.3 | 92.8% | 92.7% | (many diffs) |
| 3 | human | 3357 | HTR2B | 5-hydroxytryptamine recepto... | NM_001320758.2 | 91.1% | 91% | 0_1ins126;1304A>G |
| 4 | human | 3357 | HTR2B | 5-hydroxytryptamine recepto... | XM_005246520.4 | 75.1% | 75% | 0_1ins357;1073A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1509
- ORF length:
- 1443
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tctctcttac agagtgtctg aacttcaaag cacaattcct gagcacattt 121 tgcagagcac ctttgttcac gttatctctt ctaactggtc tggattacag acagaatcaa 181 taccagagga aatgaaacag attgttgagg aacagggaaa taaactgcac tgggcagctc 241 ttctgatact catggtgata atacccacaa ttggtggaaa tacccttgtt attctggctg 301 tttcactgga gaagaagctg cagtatgcta ctaattactt tctaatgtcc ttggcggtgg 361 ctgatttgct ggttggattg tttgtgatgc caattgccct cttgacaata atgtttgagg 421 ctatgtggcc cctcccactt gttctatgtc ctgcctggtt atttcttgac gttctctttt 481 caaccgcatc catcatgcat ctctgtgcca tttcagtgga tcgttacata gccatcaaaa 541 agccaatcca ggccaatcaa tataactcac gggctacagc attcatcaag attacagtgg 601 tgtggttaat ttcaataggc attgccattc cagtccctat taaagggata gagactgatg 661 tggacaaccc aaacaatatc acttgtgtgc tgacaaagga acgttttggc gatttcatgc 721 tctttggctc actggctgcc ttcttcacac ctcttgcaat tatgattgtc acctactttc 781 tcactatcca tgctttacag aagaaggctt acttagtcaa aaacaagcca cctcaacgcc 841 taacatggtt gactgtgtct acagttttcc aaagggatga aacaccttgc tcgtcaccgg 901 aaaaggtggc aatgctggat ggttctcgaa aggacaaggc tctgcccaac tcaggtgatg 961 aaacacttat gcgaagaaca tccacaattg ggaaaaagtc agtgcagacc atttccaacg 1021 aacagagagc ctcaaaggtc ctagggattg tgtttttcct ctttttgctt atgtggtgtc 1081 ccttctttat tacaaatata actttagttt tatgtgattc ctgtaaccaa actactctcc 1141 aaatgctcct ggagatattt gtgtggatag gctatgtttc cTCAGGAGTG AATCCTTTGG 1201 TCTACACCCT CTTCAATAAG ACATTTCGGG ATGCATTTGG CCGATATATC ACCTGCAATT 1261 ACCGGGCCAC AAAGTCAGTA AAAACTCTCA GAAAACGCTC CAGTAAGATC TACTTCCGGA 1321 ATCCAATGGC AGAGAACTCT AAGTTTTTCA AGAAACATGG AATTCGAAAT GGGATTAACC 1381 CTGCCATGTA CCAGAGTCCA ATGAGGCTCC GAAGTTCAAC CATTCAGTCT TCATCAATCA 1441 TTCTACTAGA TACGCTTCTC CTCACTGAAA ATGAAGGTGA CAAAACTGAA GAGCGAGTTA 1501 GTTATGTATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1561 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1621 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCTCCA ACTGCAGAAT GATGGACAAC 1681 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt