Construct: ORF TRCN0000466813
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002110.1_s317c1
- Derived from:
- ccsbBroadEn_08883
- DNA Barcode:
- CATTGACTTTCGGTTTTATATCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS6 (64968)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466813
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64968 | MRPS6 | mitochondrial ribosomal pro... | NM_032476.4 | 99.7% | 100% | 360A>G |
| 2 | mouse | 121022 | Mrps6 | mitochondrial ribosomal pro... | NM_080456.1 | 83.7% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 465
- ORF length:
- 375
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCCCGCTA CGAGCTGGCT TTAATCCTGA 121 AAGCCATGCA GCGGCCAGAG ACTGCTGCTA CTTTGAAACG TACGATAGAG GCCCTGATGG 181 ACAGAGGAGC AATAGTGAGG GACTTGGAAA ACCTGGGTGA ACGAGCGCTT CCTTATAGGA 241 TCTCTGCCCA CAGTCAGCAG CACAACAGAG GCGGGTATTT CTTGGTGGAT TTTTATGCAC 301 CCACCGCAGC TGTTGAAAGC ATGGTGGAGC ACTTGTCTCG AGATATAGAT GTGATTAGAG 361 GGAATATTGT CAAACACCCT CTGACCCAGG AACTAAAAGA ATGTGAAGGG ATTGTCCCAG 421 TCCCACTCGC AGAAAAATTA TATTCCACGA AGAAGAGGAA GAAGTGCCCA ACTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA CATTGACTTT CGGTTTTATA TCCTACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt