Construct: ORF TRCN0000466815

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011892.1_s317c1
Derived from:
ccsbBroadEn_07134
DNA Barcode:
AAAAACTCCAATTTCGTAGCAACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CORO2A (7464)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466815
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7464 CORO2A coronin 2A NM_003389.3 99.8% 99.8% 525G>A;1504A>G
2 human 7464 CORO2A coronin 2A NM_052820.4 99.8% 99.8% 525G>A;1504A>G
3 human 7464 CORO2A coronin 2A XM_011518986.3 99.8% 99.8% 525G>A;1504A>G
4 mouse 107684 Coro2a coronin, actin binding prot... NM_001164804.1 85.3% 85.7% (many diffs)
5 mouse 107684 Coro2a coronin, actin binding prot... NM_178893.4 82.4% 82.7% (many diffs)
6 mouse 107684 Coro2a coronin, actin binding prot... XM_011249899.2 82.4% 82.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1641
ORF length:
1575
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc atggcacccc cagtaccgga gctccaagtt ccgtcatgtc tttggcaaac
121cagccagcaa ggagaactgc tacgactccg tgcctatcac ccgcagcgtt cacgacaacc
181acttctgtgc cgtgaacccc cacttcattg cagttgtgac tgagtgtgct ggtggagggg
241ccttcctcgt catccccctg caccagacag ggaagttgga cccccactac ccaaaagtct
301gcgggcacag aggcaacgtt ttggatgtca agtggaaccc ttttgatgat tttgagatcg
361cctcctgttc tgaagatgcc acaattaaga tctggagcat ccccaagcag ctgctgacca
421ggaacctcac ggcctacagg aaggaactcg tgggccacgc gcgcagagta ggcctggtgg
481agtggcaccc cacggccgcc aacatcctct tcagtgctgg ctatgactac aaggtgatga
541tctggaacct ggatacaaag gagtctgtca tcacaagccc catgagtaca attagctgtc
601accaagatgt gatcctctcc atgtccttca acaccaacgg cagcctgttg gccaccacct
661gcaaagaccg caagattcgg gttattgacc cccgagcagg gaccgtcctc caggaggcca
721gctacaaagg gcaccgggcc agcaaagtgc tgtttctggg gaacctgaag aagctgatgt
781ccacaggcac atcccgatgg aacaaccggc aggtggcctt gtgggaccag gataacctct
841ctgtgcctct gatggaggag gacctggacg gctcctcggg cgtgctgttt cccttctatg
901acgcggacac cagcatgctc tacgtggtgg ggaagggaga tggcaacatc cgctactacg
961aggtgagcgc cgacaagcct cacctgagct acctgactga gtaccgctcc tataacccac
1021agaaggggat cggtgtcatg ccaaagagag gactcgacgt gtcctcctgc gagatcttcc
1081gcttctacaa gctgatcaca accaaaagcc tcatcgagcc catctccatg attgtgcccc
1141ggcggtcaga atcctaccaa gaggacatat accctccaac agcaggggcc cagccctccc
1201tgacggccca ggagtgGCTC AGCGGGATGA ATCGAGACCC AATCCTGGTG TCCCTTAGGC
1261CTGGCTCTGA GCTGCTGAGA CCCCACCCAC TGCCTGCAGA GAGACCTATC TTCAATTCCA
1321TGGCCCCAGC CTCACCCCGG CTCTTGAATC AGACAGAAAA GCTGGCTGCA GAAGATGGCT
1381GGAGGTCTTC CTCCCTGTTG GAGGAGAAGA TGCCAAGGTG GGCAGCAGAA CACAGGCTGG
1441AGGAGAAGAA AACCTGGCTG ACAAATGGCT TTGACGTTTT CGAATGCCCC CCACCAAAGA
1501CAGAGAATGA GTTGCTGCAG ATGTTCTACC GGCAACAGGA GGAGATCCGA AGGCTCCGGG
1561AGCTGTTGGC CCAGCGAGAG GTCCAGGCCA AACAGTTGGA ACTGGAGATC AAAAACTTGC
1621GGATGGGCTC AGAGCAGCTC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1681CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1741AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAAAA ACTCCAATTT
1801CGTAGCAACG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)