Construct: ORF TRCN0000466854
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006756.1_s317c1
- Derived from:
- ccsbBroadEn_06431
- DNA Barcode:
- CGCAGCTATACAGAGGGGCACGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFNAR2 (3455)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466854
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3455 | IFNAR2 | interferon alpha and beta r... | NM_000874.5 | 99.7% | 99.3% | 28T>G;853G>A |
| 2 | human | 3455 | IFNAR2 | interferon alpha and beta r... | NM_207584.3 | 99.7% | 99.3% | 28T>G;853G>A |
| 3 | human | 3455 | IFNAR2 | interferon alpha and beta r... | NM_001289126.1 | 71.5% | 71.2% | 28T>G;712_714delTTT;717_718ins279 |
| 4 | human | 3455 | IFNAR2 | interferon alpha and beta r... | NM_001289128.1 | 71.5% | 71.2% | 28T>G;712_714delTTT;717_718ins279 |
| 5 | human | 3455 | IFNAR2 | interferon alpha and beta r... | NM_001289125.1 | 60.4% | 54.5% | (many diffs) |
| 6 | human | 3455 | IFNAR2 | interferon alpha and beta r... | NM_207585.2 | 60.4% | 54.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1059
- ORF length:
- 993
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct tttgagccag aatgccttca tcgtcagatc acttaatttg gttctcatgg 121 tgtatatcag cctcgtgttt ggtatttcat atgattcgcc tgattacaca gatgaatctt 181 gcactttcaa gatatcattg cgaaatttcc ggtccatctt atcatgggaa ttaaaaaacc 241 actccattgt accaactcac tatacattgc tgtatacaat catgagtaaa ccagaagatt 301 tgaaggtggt taagaactgt gcaaatacca caagatcatt ttgtgacctc acagatgagt 361 ggagaagcac acacgaggcc tatgtcaccg tcctagaagg attcagcggg aacacaacgt 421 tgttcagttg ctcacacaat ttctggctgg ccatagacat gtcttttgaa ccaccagagt 481 ttgagattgt tggttttacc aaccacatta atgtgatggt gaaatttcca tctattgttg 541 aggaagaatt acagtttgat ttatctctcg tcattgaaga acagtcagag ggaattgtta 601 agaagcataa acccgaaata aaaggaaaca tgagtggaaa tttcacctat atcattgaca 661 agttaattcc aaacacgaac tactgtgtat ctgtttattt agagcacagt gatgagcaag 721 cagtaataaa gtctccctta aaatgcaccc tccTTCCACC TGGCCAGGAA TCAGAATCAG 781 CAGAATCTGC CAAAATAGGA GGAATAATTA CTGTGTTTTT GATAGCATTG GTCTTGACAA 841 GCACCATAGT GACACTGAAA TGGATTGGTT ATATATGCTT AAGAAATAGC CTCCCCAAAG 901 TCTTGAGGCA AGGTCTCACT AAGGGCTGGA ATGCAGTGGC TATTCACAGG TGCAGTCATA 961 ATGCACTACA GTCTGAAACT CCTGAGCTCA AACAGTCGTC CTGCCTAAGC TTCCCCAGTA 1021 GCTGGGATTA CAAGCGTGCA TCCCTGTGCC CCAGTGATTA CCCAACTTTC TTGTACAAAG 1081 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1141 GAACTAGTCC GTAACTTGAA AGTTTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 1201 CGACGCAGCT ATACAGAGGG GCACGCAACG CGTTAAGTCg acaatcaacc tctggattac 1261 aaaatttgtg aaagatt