Construct: ORF TRCN0000466865
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002495.1_s317c1
- Derived from:
- ccsbBroadEn_02265
- DNA Barcode:
- CTAACAAACCCCGGCATAGGGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OSBPL2 (9885)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466865
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9885 | OSBPL2 | oxysterol binding protein l... | NM_144498.3 | 100% | 100% | |
| 2 | human | 9885 | OSBPL2 | oxysterol binding protein l... | NM_014835.4 | 97.5% | 97.5% | 34_35ins36 |
| 3 | human | 9885 | OSBPL2 | oxysterol binding protein l... | NM_001363878.1 | 80.8% | 80.8% | 0_1ins276 |
| 4 | human | 9885 | OSBPL2 | oxysterol binding protein l... | NM_001278649.2 | 68.8% | 58.5% | 0_1ins276;848_849ins124;1041_1110del |
| 5 | mouse | 228983 | Osbpl2 | oxysterol binding protein-l... | NM_144500.3 | 84.9% | 89% | (many diffs) |
| 6 | mouse | 228983 | Osbpl2 | oxysterol binding protein-l... | XM_017317993.1 | 84.9% | 89% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1506
- ORF length:
- 1440
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cggagaggaa gaattctttg atgccgtcac aggctttgat tctgataact 121 cttctgggga attttcagag gcaaatcaga aagtcacggg aatgattgac ttagacacca 181 gcaaaaataa taggattggg aaaactgggg agaggccctc tcaagagaac ggaattcaga 241 aacacaggac atcgctgccg gctcccatgt tcagcagaag cgacttcagc gtgtggacca 301 tcctgaagaa gtgtgttggc ctggagctgt ccaagatcac gatgccaatc gccttcaacg 361 agcctctgag cttcttgcag cggatcacgg agtacatgga gcacgtgtac ctcatccaca 421 gggcctcctg ccagccccag cccctggaga ggatgcagtc tgtggctgct tttgctgttt 481 cggctgtggc ttcccagtgg gagaggaccg gcaaaccatt taatccactc ttgggagaaa 541 cgtatgaatt aatcagggaa gatttaggat tcagatttat atcggaacag gtcagtcacc 601 acccccccat cagtgcgttc cactcggaag gtctcaacca tgacttcctg ttccatggct 661 ccatctaccc caagctcaag ttctggggca aaagcgtgga ggcggagccc cgaggcacca 721 tcaccctgga gctgctcaaa cataatgaag cctacacctg gaccaacccc acctgctgcg 781 tccacaacgt catcatcggg aagctgtgga tagagcagta tgggacagtg gagattttaa 841 accacagaac tggacataag tgtgtgcttc actttaaacc gtgtggatta tttggaaaag 901 aacttcacaa ggtggaagga cacattcaag acaaaaacaa aaagaagctc tttatgatct 961 atggcaaatg gacggaatgt ttgtggggca tagatcctgt ttcgtatgaa tccttcaaga 1021 agcaggagag gagaggtgac cacctgagaa aggccaagct ggatgaagac TCCGGGAAGG 1081 CTGACAGCGA CGTGGCTGAC GACGTGCCTG TGGCCCAGGA GACCGTGCAG GTCATTCCTG 1141 GCAGCAAGCT GCTCTGGAGG ATCAACACCC GGCCCCCCAA CTCTGCCCAG ATGTATAATT 1201 TCACCAGTTT CACTGTGAGC CTCAACGAGC TGGAGACAGG CATGGAGAAG ACCCTGCCAC 1261 CCACGGACTG CCGCCTGCGC CCTGACATCC GCGGCATGGA GAATGGCAAC ATGGATCTGG 1321 CCAGCCAGGA GAAGGAGCGG CTGGAGGAGA AGCAGAGAGA AGCACGGAGG GAGCGGGCCA 1381 AGGAGGAGGC AGAGTGGCAG ACGAGGTGGT TCTACCCAGG CAATAACCCC TACACTGGGA 1441 CCCCCGACTG GTTGTATGCA GGGGATTACT TTGAGCGGAA TTTCTCCGAC TGCCCAGATA 1501 TCTACTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1561 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1621 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTAACAAAC CCCGGCATAG GGAAAACGCG 1681 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt