Construct: ORF TRCN0000466890
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011530.1_s317c1
- Derived from:
- ccsbBroadEn_13014
- DNA Barcode:
- AGCTAACGCTAGCCCCCCAGCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLB1L3 (112937)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466890
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 112937 | GLB1L3 | galactosidase beta 1 like 3 | XM_011542572.1 | 35% | 32.4% | (many diffs) |
2 | human | 112937 | GLB1L3 | galactosidase beta 1 like 3 | XM_024448338.1 | 32.2% | 29.5% | (many diffs) |
3 | human | 112937 | GLB1L3 | galactosidase beta 1 like 3 | XM_011542573.1 | 30.5% | 25.1% | (many diffs) |
4 | human | 112937 | GLB1L3 | galactosidase beta 1 like 3 | XM_011542574.2 | 27.1% | 24.3% | (many diffs) |
5 | human | 112937 | GLB1L3 | galactosidase beta 1 like 3 | XM_011542571.2 | 26.7% | 24.7% | (many diffs) |
6 | human | 112937 | GLB1L3 | galactosidase beta 1 like 3 | NM_001080407.3 | 22.4% | 20.7% | (many diffs) |
7 | human | 112937 | GLB1L3 | galactosidase beta 1 like 3 | XM_011542567.2 | 21.2% | 19.6% | (many diffs) |
8 | human | 112937 | GLB1L3 | galactosidase beta 1 like 3 | XM_011542566.2 | 20.9% | 19.3% | (many diffs) |
9 | human | 112937 | GLB1L3 | galactosidase beta 1 like 3 | XM_017017155.2 | 20.6% | 18.9% | (many diffs) |
10 | human | 112937 | GLB1L3 | galactosidase beta 1 like 3 | XM_011542568.2 | 19.2% | 17.6% | (many diffs) |
11 | human | 112937 | GLB1L3 | galactosidase beta 1 like 3 | XR_002957120.1 | 17.4% | (many diffs) | |
12 | human | 112937 | GLB1L3 | galactosidase beta 1 like 3 | XR_947791.2 | 15% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 549
- ORF length:
- 483
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gtatctccac aaggccctgc tgagaagagg gattgtggag cttctcttga 121 cctctgatgg tgagaaacat gtgctgagtg gccacaccaa aggagtgttg gccgccatca 181 atttgcaaaa acttcaccag gatactttca atcagcttca taaagtccag agagataagc 241 cccTTCTGAT TATGGAATAC TGGGTCGGCT GGTTCGACAG ATGGGGAGAT AAGCACCATG 301 TTAAAGATGC AAAGGAGGTT GAACATGCTG TGTCTGAATT CATCAAATAT GAGATCTCCT 361 TCAATGTATA TATGTTCCAT GGTGGAACCA ACTTTGGTTT CATGAACGGG GCCACATATT 421 TCGGGAAGCA CTCGGGCATT GTCACCAGCT ATGGCAAGTG TCGCTGGTGT AGTAGCCTCT 481 CCAGCATGGG CGGTGCTGGG GCTTTACAGA GGAGGCTCCG GAAGCCTGTT CTGGCACCAC 541 TGGGTTCTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 601 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 661 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGCTAA CGCTAGCCCC CCAGCATAAC 721 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt