Construct: ORF TRCN0000466919
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004869.1_s317c1
- Derived from:
- ccsbBroadEn_04996
- DNA Barcode:
- CTCCATATCTTCTCTAGTATATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APCDD1 (147495)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466919
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 147495 | APCDD1 | APC down-regulated 1 | NM_153000.5 | 100% | 100% | |
| 2 | human | 147495 | APCDD1 | APC down-regulated 1 | XM_011525603.2 | 66.2% | 57.4% | (many diffs) |
| 3 | mouse | 494504 | Apcdd1 | adenomatosis polyposis coli... | NM_133237.3 | 85.5% | 91.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1608
- ORF length:
- 1542
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ctggccgcgc cgcctcctgc tcagatacct gttcccggcc ctcctgcttc 121 acgggctggg agagggttct gccctccttc atccagacag caggtctcat cctaggtcct 181 tagagaaaag tgcctggagg gcttttaagg agtcacagtg ccatcacatg ctcaaacatc 241 tccacaatgg tgcaaggatc acagtgcaga tgccacctac aatcgagggc cactgggtct 301 ccacaggctg tgaagtaagg tcaggcccag agttcatcac aaggtcctac agattctacc 361 acaataacac cttcaaggcc taccaatttt attatggcag caaccggtgc acaaatccca 421 cttatactct catcatccgg ggcaagatcc gcctccgcca ggcctcctgg atcatccgag 481 ggggcacgga agccgactac cagctgcaca acgtccaggt gatctgccac acagaggcgg 541 tggccgagaa gctcggccag caggtgaacc gcacatgccc gggcttcctc gcagacgggg 601 gtccctgggt gcaggacgtg gcctatgacc tctggcgaga ggagaacggc tgtgagtgca 661 ccaaggccgt gaactttgcc atgcatgaac ttcagctcat ccgggtggag aagcagtacc 721 ttcaccacaa cctcgaccac ctggtcgagg agctcttcct tggtgacatt cacactgatg 781 ccacccagag gatgttctac cggccctcca gttaccagcc ccctctgcag aatgccaaga 841 accacgacca tgcctgcatc gcctgtcgga tcatctatcg gtcagacgag caccaccctc 901 ccatcctgcc cccaaaggca gacctgacca tcggcctgca cggggagtgg gtgagccagc 961 gctgtgaggt gcgccccgaa gtcctcttcc tcacccgcca cttcatcttc catgacaaca 1021 acaacacctg ggagggccac tactaccact actcagaccc ggtgtgcaag caccccacct 1081 tctccatcta cgcccggggc cgctacagcc gcggcgtcct ctcgtccagg gtcatgggag 1141 gcaccgagtt cgtgttcaaa gtgaatcaca tgaaggtcac ccccatggat gcggccacag 1201 cctcactgct caacgtcttc aacgggAATG AGTGCGGGGC CGAGGGCTCC TGGCAGGTGG 1261 GCATCCAGCA GGATGTGACC CACACCAATG GCTGCGTGGC CCTGGGCATC AAACTACCTC 1321 ACACGGAGTA CGAGATCTTC AAAATGGAAC AGGATGCCCG GGGGCGCTAT CTGCTGTTCA 1381 ACGGTCAGAG GCCCAGCGAC GGGTCCAGCC CAGACAGGCC AGAGAAGAGA GCCACGTCCT 1441 ACCAGATGCC CTTGGTCCAG TGTGCCTCCT CTTCGCCGAG GGCAGAGGAC CTCGCAGAAG 1501 ACAGTGGAAG CAGCCTGTAT GGCCGGGCCC CTGGGAGGCA CACCTGGTCC CTGCTGCTGG 1561 CTGCACTTGC CTGCCTTGTC CCTCTGCTGC ATTGGAACAT CCGCAGATAC CCAACTTTCT 1621 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1681 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1741 GTGGAAAGGA CGACTCCATA TCTTCTCTAG TATATTAACG CGTTAAGTCg acaatcaacc 1801 tctggattac aaaatttgtg aaagatt