Construct: ORF TRCN0000466934
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004465.1_s317c1
- Derived from:
- ccsbBroadEn_01887
- DNA Barcode:
- AATTGTACTGGTACGATGCTTTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACOX2 (8309)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466934
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8309 | ACOX2 | acyl-CoA oxidase 2 | NM_003500.4 | 100% | 100% | |
| 2 | human | 8309 | ACOX2 | acyl-CoA oxidase 2 | XM_005265505.1 | 100% | 100% | |
| 3 | human | 8309 | ACOX2 | acyl-CoA oxidase 2 | XM_017007302.2 | 89.7% | 89.7% | 0_1ins210 |
| 4 | human | 8309 | ACOX2 | acyl-CoA oxidase 2 | XM_024453786.1 | 89.7% | 89.7% | 0_1ins210 |
| 5 | human | 8309 | ACOX2 | acyl-CoA oxidase 2 | XM_006713340.3 | 85.2% | 84.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2109
- ORF length:
- 2043
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cagcccagtg caccgagtgt cattggggga tacctggagc aggcaaatgc 121 accccgacat agagagcgag aggtatatgc agtcctttga cgtggaacgg ctcaccaaca 181 tccttgatgg aggtgcccag aacactgcac tccgcaggaa agttgagagc atcatccaca 241 gttacccgga gtttagctgt aaggacaatt atttcatgac ccagaatgag cgttataagg 301 ctgccatgcg gagggcattc cacatccggt tgatagctcg gcgcctgggt tggttagaag 361 atggtcgtga attaggctac gcttacagag ccctttctgg agacgtggcc ttaaatatac 421 acagagtctt cgtgagagcc ctcaggagcc tgggctcaga ggagcagatt gccaaatggg 481 acccactctg caaaaacatc cagatcatcg caacgtatgc acagacagag ttgggacatg 541 ggacatatct tcagggcctg gagactgaag ccacctatga cgcagccacc caggagtttg 601 tgatacacag ccccacgctg actgccacca aatggtggcc tggagacttg ggacggtcag 661 ccacccatgc cctggtccag gcccagctga tctgctcagg agccaggcgg ggcatgcacg 721 cttttattgt gccaatccgg agtcttcagg accacacccc actgccagga atcatcattg 781 gggacatcgg acccaagatg gactttgatc aaacagacaa tggcttcctg cagctgaacc 841 atgtgcgggt ccccagggag aacatgctga gtcgctttgc acaggtcttg ccagatggca 901 cctacgtcaa actcggtaca gcacagagca actaccttcc catggtggtg gtgcgggtgg 961 agctgctgtc aggggagatc ctccctatac tgcagaaggc ctgtgtcatc gccatgcgct 1021 actcggtcat ccgccgccaa tcccggctcc ggcccagtga cccagaggca aaggtcctgg 1081 actaccagac acaacagcag aaactctttc ctcagctggc catcagttat gccttccatt 1141 tcctggcagt cagcctcttg gagttcttcc agcactccta cactgccatt ctgaaccaag 1201 acttcagctt cctgcctgag ctccacgcac tgagcacggg catgaaggcc atgatgtcag 1261 aattctgcac ccagggagct gagatgtgcc gcagggcctg tggcggacat ggctactcaa 1321 agctgagtgg cctgccatca ctggtcacca aattgtcggc ctcctgtacc tacgagggtg 1381 agaacacagt gctctacctg caggtggcca ggttcctggt gaagagctac ctgcagactc 1441 agatgtcccc tggctccacg ccacagagat ctctctctcc atctgtcgca tatctcaccg 1501 cacctgacct ggccaggtgt ccagcccaga gggcagccga cttcctctgc ccggagctct 1561 acaccacggc ctgggcacat gtggcagtaa ggctcataaa ggactcagtg cagcatttac 1621 agaccctgac gcaatccgga gctgaccagc acgaggcttg gaaccagacc actgtcatac 1681 accTCCAGGC TGCTAAGGTG CACTGCTACT ATGTCACTGT GAAGGGTTTT ACAGAAGCTC 1741 TGGAGAAACT AGAAAATGAA CCAGCGATTC AGCAGGTGCT CAAGCGCCTC TGTGACCTCC 1801 ATGCCATACA TGGAATCTTG ACTAACTCGG GTGACTTTCT CCATGACGCC TTCCTGTCTG 1861 GTGCCCAAGT GGACATGGCA AGAACAGCCT ACCTGGACCT GCTCCGCCTG ATCCGGAAGG 1921 ATGCCATCCT GTTAACTGAT GCTTTTGACT TCACCGATCA GTGTTTAAAT TCAGCACTTG 1981 GCTGTTATGA TGGAAACGTC TACGAACGCC TGTTCCAGTG GGCTCAGAAG TCACCAACCA 2041 ATACTCAGGA GAACCCTGCC TATGAGGAAT ATATAAGACC ACTTTTACAA AGTTGGAGAT 2101 CCAAGCTATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2161 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2221 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAATTGT ACTGGTACGA TGCTTTCGAC 2281 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt