Construct: ORF TRCN0000466948

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009267.1_s317c1
Derived from:
ccsbBroadEn_01466
DNA Barcode:
TTTAAAGCAATGTTTATTCGGAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RRM2 (6241)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466948
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6241 RRM2 ribonucleotide reductase re... NM_001034.4 100% 100%
2 human 6241 RRM2 ribonucleotide reductase re... NM_001165931.1 86.6% 86.6% 1_180del
3 human 6241 RRM2 ribonucleotide reductase re... NR_164157.1 51.5% (many diffs)
4 mouse 20135 Rrm2 ribonucleotide reductase M2 NM_009104.2 88% 91.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1233
ORF length:
1167
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct ctccctccgt gtcccgctcg cgcccatcac ggacccgcag cagctgcagc
121tctcgccgct gaaggggctc agcttggtcg acaaggagaa cacgccgccg gccctgagcg
181ggacccgcgt cctggccagc aagaccgcga ggaggatctt ccaggagccc acggagccga
241aaactaaagc agctgccccc ggcgtggagg atgagccgct gctgagagaa aacccccgcc
301gctttgtcat cttccccatc gagtaccatg atatctggca gatgtataag aaggcagagg
361cttccttttg gaccgccgag gaggtggacc tctccaagga cattcagcac tgggaatccc
421tgaaacccga ggagagatat tttatatccc atgttctggc tttctttgca gcaagcgatg
481gcatagtaaa tgaaaacttg gtggagcgat ttagccaaga agttcagatt acagaagccc
541gctgtttcta tggcttccaa attgccatgg aaaacataca ttctgaaatg tatagtcttc
601ttattgacac ttacataaaa gatcccaaag aaagggaatt tctcttcaat gccattgaaa
661cgatgccttg tgtcaagaag aaggcagact gggccttgcg ctggattggg gacaaagagg
721ctacctatgg tgaacgtgtt gtagcctttg ctgcagtgga aggcattttc ttttccggtt
781cttttgcgtc gatattctgg ctcaagaaac gaggactgat gcctggcctc acattttcta
841atgaacttat tagcagagat gagggtttac actgtgattt tgcttgccTG ATGTTCAAAC
901ACCTGGTACA CAAACCATCG GAGGAGAGAG TAAGAGAAAT AATTATCAAT GCTGTTCGGA
961TAGAACAGGA GTTCCTCACT GAGGCCTTGC CTGTGAAGCT CATTGGGATG AATTGCACTC
1021TAATGAAGCA ATACATTGAG TTTGTGGCAG ACAGACTTAT GCTGGAACTG GGTTTTAGCA
1081AGGTTTTCAG AGTAGAGAAC CCATTTGACT TTATGGAGAA TATTTCACTG GAAGGAAAGA
1141CTAACTTCTT TGAGAAGAGA GTAGGCGAGT ATCAGAGGAT GGGAGTGATG TCAAGTCCAA
1201CAGAGAATTC TTTTACCTTG GATGCTGACT TCTACCCAAC TTTCTTGTAC AAAGTGGTTG
1261ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1321GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT
1381TAAAGCAATG TTTATTCGGA ACACGCGTTA AGTCgacaat caacctctgg attacaaaat
1441ttgtgaaaga tt

Download FASTA (ORF) (Full)