Construct: ORF TRCN0000466953
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000411.1_s317c1
- Derived from:
- ccsbBroadEn_14361
- DNA Barcode:
- TAAAGTCTGCATATGCCAAGCTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNAP47 (116841)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466953
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116841 | SNAP47 | synaptosome associated prot... | NM_001323930.1 | 52.8% | 52.7% | 1_591del;1071C>T;1253T>A |
| 2 | human | 116841 | SNAP47 | synaptosome associated prot... | NM_001323931.1 | 52.8% | 52.7% | 1_591del;1071C>T;1253T>A |
| 3 | human | 116841 | SNAP47 | synaptosome associated prot... | NM_001323932.1 | 52.8% | 52.7% | 1_591del;1071C>T;1253T>A |
| 4 | human | 116841 | SNAP47 | synaptosome associated prot... | NM_001323933.1 | 52.8% | 52.7% | 1_591del;1071C>T;1253T>A |
| 5 | human | 116841 | SNAP47 | synaptosome associated prot... | NM_001323934.1 | 52.8% | 52.7% | 1_591del;1071C>T;1253T>A |
| 6 | human | 116841 | SNAP47 | synaptosome associated prot... | XM_006711734.3 | 50.8% | 50.8% | 1_639del;1119C>T;1301T>A |
| 7 | human | 116841 | SNAP47 | synaptosome associated prot... | NM_053052.3 | 47.7% | 47.6% | 1_726del;1206C>T;1388T>A |
| 8 | human | 116841 | SNAP47 | synaptosome associated prot... | XM_017000231.2 | 47.7% | 47.6% | 1_726del;1206C>T;1388T>A |
| 9 | human | 116841 | SNAP47 | synaptosome associated prot... | XM_017000232.1 | 47.7% | 47.6% | 1_726del;1206C>T;1388T>A |
| 10 | human | 116841 | SNAP47 | synaptosome associated prot... | NR_136654.1 | 41.7% | (many diffs) | |
| 11 | human | 116841 | SNAP47 | synaptosome associated prot... | NM_001323935.1 | 40.6% | 38.3% | (many diffs) |
| 12 | human | 116841 | SNAP47 | synaptosome associated prot... | NR_136656.1 | 36.3% | (many diffs) | |
| 13 | human | 116841 | SNAP47 | synaptosome associated prot... | NR_136657.2 | 32.7% | (many diffs) | |
| 14 | human | 116841 | SNAP47 | synaptosome associated prot... | NR_136655.1 | 26.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 732
- ORF length:
- 666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cagttgtgaa ccctttggga aagaagggat actgataaaa attcctgctg 121 ttatttccca cagaacagag tctcacgtta aaccagggag gctcaccgtc cttgtgtctg 181 ggttggaaat acatgactcc agttctttgc tcatgcacag gtttgaaaga gaagacgtgg 241 acgacatcaa ggtccactca ccttacgaaa ttagcatccg ccagcggttt attggaaagc 301 cagacatggc ctatcgtttg atatctgcca agatgccaga ggttatcccc attTTAGAAG 361 TGCAGTTCAG CAAGAAGATG GAGCTGTTAG AAGATGCATT GGTGCTCAGA AGCGCAAGAA 421 CCTCTTCCCC CGCAGAGAAG AGCTGCTCAG TCTGGCATGC AGCATCTGGG CTGATGGGCC 481 GTACCCTGCA CCGTGAGCCA CCCGCAGGAG ACCAGGAGGG CACAGCACTG CACCTGCAGA 541 CAAGTCTGCC AGCCCTTTCT GAGGCAGATA CCCAGGAACT AACCCAGATC CTGAGGAGGA 601 TGAAGGGGCT GGCCCTGGAG GCCGAGAGTG AGCTGGAGAG ACAAGACGAA GCCCTGGATG 661 GCGTTGCAGC AGCTGTGGAC AGGGCAACCT TGACCATCGA CAAGCACAAC AGGCGGATGA 721 AGAGGCAGAC CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 781 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 841 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATAA AGTCTGCATA TGCCAAGCTG 901 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t