Construct: ORF TRCN0000466966
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007677.1_s317c1
- Derived from:
- ccsbBroadEn_01370
- DNA Barcode:
- GACGGAGTTGGTCTGACTGTAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAD51D (5892)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466966
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5892 | RAD51D | RAD51 paralog D | NM_002878.3 | 100% | 100% | |
| 2 | human | 5892 | RAD51D | RAD51 paralog D | NM_001142571.2 | 89.9% | 83.4% | (many diffs) |
| 3 | human | 5892 | RAD51D | RAD51 paralog D | NM_133629.3 | 65.8% | 65.8% | 142_143ins336 |
| 4 | human | 100529207 | RAD51L3-RFFL | RAD51L3-RFFL readthrough | NR_037714.1 | 15.5% | (many diffs) | |
| 5 | human | 5892 | RAD51D | RAD51 paralog D | NR_037711.2 | 8.7% | 1_145del;287_288ins119;1011_9745del | |
| 6 | human | 5892 | RAD51D | RAD51 paralog D | NR_037712.2 | 7.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1050
- ORF length:
- 984
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cgtgctcagg gtcggactgt gccctggcct taccgaggag atgatccagc 121 ttctcaggag ccacaggatc aagacagtgg tggacctggt ttctgcagac ctggaagagg 181 tagctcagaa atgtggcttg tcttacaagg ccctggttgc cctgaggcgg gtgctgctgg 241 ctcagttctc ggctttcccc gtgaatggcg ctgatctcta cgaggaactg aagacctcca 301 ctgccatcct gtccactggc attggcagtc ttgataaact gcttgatgct ggtctctata 361 ctggagaagt gactgaaatt gtaggaggcc caggtagcgg caaaactcag gtatgtctct 421 gtatggcagc aaatgtggcc catggcctgc agcaaaacgt cctatatgta gattccaatg 481 gagggctgac agcttcccgc ctcctccagc tgcttcaggc taaaacccag gatgaggagg 541 aacaggcaga agctctccgg aggatccagg tggtgcatgc atttgacatc ttccagatgc 601 tggatgtgct gcaggagctc cgaggcactg tggcccagca ggtgactggt tcttcaggaa 661 ctgtgaaggt ggtggttgtg gactcggtca ctgcggtggt ttcccCACTT CTGGGAGGTC 721 AGCAGAGGGA AGGCTTGGCC TTGATGATGC AGCTGGCCCG AGAGCTGAAG ACCCTGGCCC 781 GGGACCTTGG CATGGCAGTG GTGGTGACCA ACCACATAAC TCGAGACAGG GACAGCGGGA 841 GGCTCAAACC TGCCCTCGGA CGCTCCTGGA GCTTTGTGCC CAGCACTCGG ATTCTCCTGG 901 ACACCATCGA GGGAGCAGGA GCATCAGGCG GCCGGCGCAT GGCGTGTCTG GCCAAATCTT 961 CCCGACAGCC AACAGGTTTC CAGGAGATGG TAGACATTGG GACCTGGGGG ACCTCAGAGC 1021 AGAGTGCCAC ATTACAGGGT GATCAGACAT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1081 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1141 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGACGG 1201 AGTTGGTCTG ACTGTAAATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1261 tgaaagatt