Construct: ORF TRCN0000466979
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013976.1_s317c1
- Derived from:
- ccsbBroadEn_03057
- DNA Barcode:
- ACGCTGAGGCCAGTGTCCTACTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOB1 (28987)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466979
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28987 | NOB1 | NIN1 (RPN12) binding protei... | NM_014062.3 | 100% | 100% | |
| 2 | human | 28987 | NOB1 | NIN1 (RPN12) binding protei... | NR_074074.1 | 62.2% | 1_58del;253_254ins131;1164_1644del | |
| 3 | mouse | 67619 | Nob1 | NIN1/RPN12 binding protein ... | NM_026277.3 | 84.4% | 88.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1302
- ORF length:
- 1236
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tccagtggag cacgttgtgg cggatgctgg ggctttcctg cggcatgcgg 121 ctctgcagga catcgggaag aacatttaca ccatccggga ggtggtcact gagattcggg 181 acaaggccac acgcaggcgg ctcgctgtcc tgccctacga gctgcggttc aaggagccct 241 taccggaata cgtgcggctg gtgactgagt tttcaaagaa aacaggagac taccccagcc 301 tctctgccac ggacatccaa gtgcttgcac tcacatacca gttggaagca gagtttgttg 361 gggtgtctca cctaaaacaa gaaccacaga aggttaaggt gagctcatcg attcagcacc 421 cagaaacacc tctgcacatt tctggtttcc atctgcccta caagcctaaa cccccacaag 481 aaacagaaaa aggacactca gcttgtgagc ctgagaacct ggaatttagt tccttcatgt 541 tctggagaaa ccctttgccc aacatcgatc atgaactgca ggagctgctg attgacagag 601 gtgaggacgt tccaagtgag gaggaggagg aggaagaaaa cgggtttgaa gacagaaaag 661 atgacagcga tgacgacggg ggtggctgga taacccccag taacatcaag cagatccagc 721 aggagctgga gcagtgtgac gtccccgagg acgtgcgggt tggctgcctg accacagact 781 tcgccatgca gaatgttctg ctgcagatgg ggctgcacgt gctggcggtg aacggcatgc 841 tgattcgtga ggcccggagc tacatcttgc gctgccatgg ctgtttcaag acaacgtctg 901 acatgagccg agtgttctgc tcacactgtg ggaacaagac cctgaagaaa gtgtccgtga 961 ccgtcagcga cgacggcacc ctgcacatgc acttctcccg caaccccaag gtgctgaacc 1021 cccgcggcct ccggtactcg cttcccactc ccaaaggggg caaatacgcc atcaaccccc 1081 atctcaccga ggatcagcgc ttccctcagc tgcgactctc ccaaaaggcc aggcagaaaa 1141 ccaacgtgtt cgcccctgac TACATCGCCG GGGTGTCACC CTTTGTCGAG AATGACATCT 1201 CCAGCCGCTC AGCTACCCTG CAGGTCCGGG ACAGCACCTT GGGAGCTGGG CGGAGACGCT 1261 TAAATCCCAA CGCTTCCAGA AAGAAGTTTG TGAAGAAAAG GTGCCCAACT TTCTTGTACA 1321 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1381 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1441 AGGACGAACG CTGAGGCCAG TGTCCTACTA CACGCGTTAA GTCgacaatc aacctctgga 1501 ttacaaaatt tgtgaaagat t