Construct: ORF TRCN0000466987
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016255.1_s317c1
- Derived from:
- ccsbBroadEn_04208
- DNA Barcode:
- TAGGGTTTGACCTTCGTCGAGAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SP6 (80320)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466987
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80320 | SP6 | Sp6 transcription factor | NM_001258248.2 | 100% | 100% | |
| 2 | human | 80320 | SP6 | Sp6 transcription factor | NM_199262.3 | 100% | 100% | |
| 3 | human | 80320 | SP6 | Sp6 transcription factor | XM_006722115.3 | 100% | 100% | |
| 4 | mouse | 83395 | Sp6 | trans-acting transcription ... | NM_001363230.1 | 90.7% | 96.2% | (many diffs) |
| 5 | mouse | 83395 | Sp6 | trans-acting transcription ... | NM_031183.3 | 90.7% | 96.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1197
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctaaccgct gtctgcggct ctctgggcag ccagcacacg gaagcgccgc 121 acgcctcccc gccgcgcctc gacctgcagc ctctccaaac ttaccagggc cacacgagcc 181 ctgaggccgg ggactacccc tccccgctgc agcctggaga gctgcagagc ctcccgctgg 241 gcccggaggt ggacttctcg cagggctatg agctgccagg ggcctcctcg cgggtaacct 301 gcgaggacct ggaaagcgac agtcccttgg ccccgggccc cttttccaag ctcctgcagc 361 cggacatgtc acaccattat gaatcgtggt tcaggccgac tcacccaggc gcggaggatg 421 gctcgtggtg ggaccttcat ccgggcacca gctggatgga cctcccccac actcagggcg 481 cgctgacctc acctggccac ccgggggcgc ttcaggcggg cttggggggc tacgtcggag 541 accaccagct ttgtgccccg ccaccccacc cgcatgcgca ccacctcctt ccagctgccg 601 gagggcagca tctcctaggg ccgcccgacg gggctaaggc cttggaagta gccgccccgg 661 agtctcaagg gctggattcc agcctggacg gggcggcgcg tcccaaaggc tcccggcggt 721 cggtgccccg cagctcaggc cagaccgtct gtcgctgccc caactgtctg gaggcggagc 781 gactgggggc tccatgtggg cccgatgggg gcaagaagaa gcatttgcac aactgccaca 841 tcccgggctg cgggaaagcc TACGCCAAGA CGTCGCACCT GAAGGCGCAC CTGCGCTGGC 901 ACAGCGGCGA CCGTCCCTTC GTGTGCAACT GGCTCTTCTG CGGCAAGCGC TTCACGCGCT 961 CGGACGAGCT GCAGCGCCAC CTCCAGACCC ACACCGGCAC CAAGAAGTTC CCCTGTGCAG 1021 TCTGCAGCCG CGTCTTCATG CGCAGCGACC ACCTGGCCAA GCACATGAAA ACCCACGAGG 1081 GCGCCAAGGA GGAGGCGGCT GGGGCGGCCT CGGGAGAGGG CAAGGCCGGC GGCGCAGTGG 1141 AGCCCCCCGG GGGCAAAGGC AAACGCGAGG CCGAGGGCAG CGTGGCTCCC TCCAACTTGC 1201 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GATAGGGTTT GACCTTCGTC GAGAGGACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt