Transcript: Mouse NM_031183.3

Mus musculus trans-acting transcription factor 6 (Sp6), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-02-28
Taxon:
Mus musculus (mouse)
Gene:
Sp6 (83395)
Length:
3376
CDS:
100..1230

Additional Resources:

NCBI RefSeq record:
NM_031183.3
NBCI Gene record:
Sp6 (83395)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_031183.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000233991 CGGACATGTCACACCACTATG pLKO_005 392 CDS 100% 10.800 15.120 N Sp6 n/a
2 TRCN0000082181 CGCTAAGACGTCGCATCTGAA pLKO.1 894 CDS 100% 4.950 6.930 N Sp6 n/a
3 TRCN0000233992 ATACGCTAAGACGTCGCATCT pLKO_005 891 CDS 100% 4.050 5.670 N Sp6 n/a
4 TRCN0000233993 ACCTAGCTTGGAGGGTTATTA pLKO_005 1758 3UTR 100% 15.000 10.500 N Sp6 n/a
5 TRCN0000082182 CACCTGGCCAAACACATGAAA pLKO.1 1081 CDS 100% 5.625 3.938 N Sp6 n/a
6 TRCN0000082178 GCCTGCTCTTTATCAAATCTA pLKO.1 3202 3UTR 100% 5.625 3.938 N Sp6 n/a
7 TRCN0000233990 TAACCTGCGAGGACCTGGAAA pLKO_005 326 CDS 100% 4.950 3.465 N Sp6 n/a
8 TRCN0000017803 GCATTTGCACAACTGCCACAT pLKO.1 852 CDS 100% 4.050 2.835 N SP6 n/a
9 TRCN0000082180 GCCGGACATGTCACACCACTA pLKO.1 390 CDS 100% 1.350 0.945 N Sp6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_031183.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04208 pDONR223 100% 90.7% 96.2% None (many diffs) n/a
2 ccsbBroad304_04208 pLX_304 0% 90.7% 96.2% V5 (many diffs) n/a
3 TRCN0000466987 TAGGGTTTGACCTTCGTCGAGAGG pLX_317 30.1% 90.7% 96.2% V5 (many diffs) n/a
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