Construct: ORF TRCN0000467012
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018971.2_s317c1
- Derived from:
- ccsbBroadEn_14943
- DNA Barcode:
- GACCTTCGTGTCCCGTTGGGCACG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- IKBKE (9641)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467012
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9641 | IKBKE | inhibitor of nuclear factor... | NM_014002.4 | 99.2% | 28.6% | (many diffs) |
| 2 | human | 9641 | IKBKE | inhibitor of nuclear factor... | XM_005273356.2 | 99.2% | 28.6% | (many diffs) |
| 3 | human | 9641 | IKBKE | inhibitor of nuclear factor... | NM_001193322.2 | 90.9% | 31.2% | (many diffs) |
| 4 | human | 9641 | IKBKE | inhibitor of nuclear factor... | NM_001193321.2 | 87.4% | 17% | (many diffs) |
| 5 | human | 9641 | IKBKE | inhibitor of nuclear factor... | XM_017002868.1 | 82.2% | 12% | (many diffs) |
| 6 | human | 9641 | IKBKE | inhibitor of nuclear factor... | XR_922002.2 | 65.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 837
- ORF length:
- 771
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gagcacagcc aattacctgt ggcacacaga tgacctgctg gggcaggggg 121 ccactgccag tgtgtacaag gcccgcaaca agaaatccgg agagctggtt gctgtgaagg 181 tcttcaacac taccagctac ctgcggcccc gcgaggtgca ggtgagggag tttgaggtcc 241 tgcggaagct gaaccaccag aacatcgtca agctctttgc ggtggaggag acgggcggaa 301 gccggcagaa ggtactggtg atggagtact gctccagtgg gagcctgctg agtgtgctgg 361 agagccctga gaatgccttt gggctgcctg aggatgagtt cctggtggtg ctgcgctgtg 421 tggtggccgg catgaaccac ctgcgggaga acggcattgt gcatcgcgac atcaagccgg 481 ggaacatcat gcgcctcgta ggggaggagg ggcagagcat ctacaagctg acagacttcg 541 gcgctgcccg ggagctggat gatgatgaga agttcgtctc ggtctatggg actgaggagt 601 acctgcatcc cgacatgtat gagcggggcg gtgcttcgaa agccccagca aaaagcgttc 661 ggggtgactg tggatctctg gagcattgga gtgaccttgt accatgcagc cactggcagc 721 ctgcccttca tcccctttgg tgggccacgg cggaacaagg agatcatgta ccggatcaca 781 cggagaagcc ggctggggcc attgcagtgc ccagaggcgg gagacggccc ctggagtgag 841 ctacaccctc cccatcacct gccagctgtc actggggctg cagagccagc tggtgcccat 901 cctggccaac atcctggagg tggagcaggc caagtgctgg ggcttcgacc agttctttgc 961 ggagaccagt gacatcctgc agcgagttgt cgtccatgtc ttctccctgt cccaggcagt 1021 cctgcaccac atctatatcc atgcccacaa cacgatagcc attttccagg aggccgtgca 1081 caagcagacc agtgtggccc cccgacacca ggagtacctc tttgagggtc acctctgtgt 1141 cctcgagccc agcgtctcag cacagcacat cgcccacacg acggcaagca gccccctgac 1201 cctcttcagc acagccatcc ctaaggggct ggccttcagg gaccctgctc tggacgtccc 1261 caagttcgtc cccaaagtgg acctgcaggc ggattacaac actgccaagg gcgtgttggg 1321 cgccggctac caggccctgc ggctggcacg ggccctgctg gatgggcagg agctaatgtt 1381 tcgggggctg cacggggtca tggaggtgct ccaggcccac atgcagaccg actctggaag 1441 tggcaaggac atccctcctt tttccctcag cagcacctgg gaactgagag gttcagcagc 1501 gtggctggaa cgcntgagat ccaggaactg aaggcggctg cagaactgag gtccaggctg 1561 cggactctag cggaggtcct ctccagatgc tcccaaaata tcacggagac ccaggagagc 1621 ctgagcagcc tgaaccggga gctggtgaag agccgggatc aggtacatga ggacagaagc 1681 atccagcaga ttcagtgctg tttggacaag atgaacttca tctacaaaca gttcaagaag 1741 tctaggatga ggccagggct tggctacaac gaggagcaga ttcacaagct ggataaggtg 1801 aatttcagtc atttagccaa aagactcctg caggtgttcc aggaggagtg cgtgcagaag 1861 tatcaagcgt ccTTAGTCAC ACACGGCAAG AGGATGAGGG TGGTGCACGA GACCAGGAAC 1921 CACCTGCGCC TGGTTGGCTG TTCTGTGGCT GCCTGTAACA CAGAAGCCCA GGGGGTCCAG 1981 GAGAGTCTCA GCAAGCTCCT GGAAGAGCTA TCTCACCAGC TCCTTCAGGA CCGAGCAAAG 2041 GGGGCTCAGG CCTCGCCGCC TCCCATAGCT CCTTACCCCA GCCCTACACG AAAGGACCTG 2101 CTTCTCCACA TGCAAGAGCT CTGCGAGGGG ATGAAGCTGC TGGCATCTGA CCTCCTGGAC 2161 AACAACCGCA TCATCGAACG GCTAAATAGA GTCCCAGCAC CTCCTGATGT CTTGCCAACT 2221 TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT 2281 TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA 2341 TCTTGTGGAA AGGACGAGAC CTTCGTGTCC CGTTGGGCAC GACGCGTTAA GTCgacaatc 2401 aacctctgga ttacaaaatt tgtgaaagat t