Construct: ORF TRCN0000467017
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011457.3_s317c1
- Derived from:
- ccsbBroadEn_06772
- DNA Barcode:
- AACAATATACGTGGATATGACGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKACG (5568)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467017
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5568 | PRKACG | protein kinase cAMP-activat... | NM_002732.3 | 99.8% | 99.7% | 12C>T;1033G>C |
| 2 | human | 5566 | PRKACA | protein kinase cAMP-activat... | NM_002730.4 | 85.2% | 82.9% | (many diffs) |
| 3 | human | 5566 | PRKACA | protein kinase cAMP-activat... | NM_207518.3 | 82.9% | 80.6% | (many diffs) |
| 4 | human | 5566 | PRKACA | protein kinase cAMP-activat... | XM_017026948.1 | 72.5% | 70% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1119
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg caacgctccc gccaagaagg acaccgagca ggaggagagc gtgaacgagt 121 tcctagccaa agccagagga gatttcctct acagatgggg aaaccccgct caaaacaccg 181 ccagctcgga tcagttcgaa cggctcagga cgctgggcat gggctccttc gggcgggtga 241 tgctggtgag gcaccaggag accggcggcc actacgccat gaagatcctc aacaagcaga 301 aggtggtgaa gatgaagcag gtcgagcaca tactgaacga gaagcgcatc ctgcaggcga 361 tcgactttcc gttcctcgtc aagctccagt tctcctttaa ggacaactcc tacctgtacc 421 tggtgatgga gtacgtgccg ggtggggaga tgttctcccg cctacagcgc gtcggaaggt 481 ttagcgagcc ccatgcctgt ttctatgccg cccaggtcgt cctggccgtc cagtacctac 541 actcgctcga cctcatccac cgcgacctga agcccgagaa tctcctcatc gaccagcagg 601 gctacctgca ggtgacggac ttcggtttcg ccaagcgcgt gaagggccgc acttggacct 661 tgtgcgggac cccagagtac ctggcccccg agatcatcct gagcaaaggc tacaacaagg 721 ccgtggactg gtgggcccta ggggtgctca tctatgagat ggccgtgggc ttcccaccct 781 tctacgccga ccagcccatc cagatctacg agaagatcgt cTCTGGGAGG GTGCGGTTTC 841 CCTCCAAACT CAGCTCTGAC CTCAAGCATC TGCTGCGGAG CCTGCTGCAG GTGGACCTCA 901 CCAAGCGCTT CGGAAACCTC AGGAACGGGG TTGGCGACAT CAAGAACCAC AAGTGGTTCG 961 CCACAACCAG CTGGATCGCC ATCTATGAGA AGAAGGTGGA AGCTCCCTTC ATCCCGAAGT 1021 ACACAGGCCC TGGGGATGCC AGTAACTTTG ACGACTACGA GGAGGAAGAG CTCCGGATCT 1081 CCATCAATGA GAAGTGTCCC AAGGAGTTTT CTGAGTTTTA CCCAACTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGAAACAAT ATACGTGGAT ATGACGCAAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt