Construct: ORF TRCN0000467018
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007370.1_s317c1
- Derived from:
- ccsbBroadEn_01796
- DNA Barcode:
- ACGCATAGGCGATTCACTTTACGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ZNF23 (7571)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467018
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7571 | ZNF23 | zinc finger protein 23 | NM_001304492.2 | 100% | .9% | |
| 2 | human | 7571 | ZNF23 | zinc finger protein 23 | NM_145911.3 | 100% | .9% | |
| 3 | human | 7571 | ZNF23 | zinc finger protein 23 | NM_001304493.2 | 90.9% | 1% | 0_1ins174 |
| 4 | human | 7571 | ZNF23 | zinc finger protein 23 | NM_001304494.2 | 90.9% | 1% | 0_1ins174 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 58
- ORF end:
- 124
- ORF length:
- 66
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaatg 61 ttggcatgct ggagaattat gggaatgtgg cctccctggg atttccactt ctcaaacctg 121 ctgtgatctc acaactggag ggaggaagtg agctgggggg ctcatctcca ctggctgcag 181 gaacaggcct ccagggcctc cagactgata ttcagactga caatgatttg acaaaggaaa 241 tgtatgaagg aaaagagaat gtatcatttg aacttcaaag agacttttcc caggaaacag 301 acttttcaga agcctctctt ctagagaaac aacaggaagt ccactcagca ggaaatataa 361 agaaggagaa gagcaacacc attgatggaa cagtgaaaga tgagacaagc cccgtggagg 421 agtgtttttt tagtcaaagt tcaaactcat atcagtgtca taccatcact ggagagcagc 481 cctctgggtg tacaggattg gggaaatcca tcagctttga tacaaaactc gtgaagcatg 541 aaataattaa ttctgaggaa agacctttca aatgtgaaga attagtagag ccctttaggt 601 gtgactctca acttattcaa catcaagaga acaacactga ggaaaagcct tatcagtgtt 661 cggagtgtgg caaagctttc agcattaatg agaaattaat ttggcatcag agacttcaca 721 gtggggagaa acccttcaaa tgtgtggagt gtgggaaaag cttcagctac agttcccatt 781 atatcacaca tcagacaatc cacagtgggg agaagcccta tcagtgtaag atgtgtggga 841 aggccttcag tgttaatgga agcctaagta ggcatcagag aatccatacg ggagagaagc 901 cctatcagtg caaggaatgt ggaaatggct tcagctgtag ttctgcatat attacacatc 961 agagagtcca cactggagag aaaccttacg agtgtaatga ctgtgggaaa gcgttcaatg 1021 ttaatgcaaa attaattcaa catcagagaa tccatactgg agagaaacct tatgaatgta 1081 atgaatgtgg aaaaggcttc aggtgcagct cccagcttag gcagcatcag agcatccaca 1141 caggagaaaa gccctatcag tgtaaagagt gtggaaaagg cttcaataat aatacaaaac 1201 tcattcagca tcagagaatc cacacaggtg agaaacccta tgaatgcact gaatgtggaa 1261 aagccttcag tgtcaaaggg aagttaatcc aacaccagag aattcacaca ggcgagaaac 1321 cctatgagtg taatgaatgc gggaaagcct tcagatgtaa ctcccaattt cggcagcatc 1381 tgagaattca cactggggag aagccctatg agtgtaatga gtgtggaaag gccttcagcg 1441 ttaatgggaa actaatgcgg catcagagaa ttcacactgg ggagaaacct tttgaatgta 1501 atgagtgtgg gagatgcttt acttctaaaa gaaacctact tgatcatcac cgaatccata 1561 ctggagaaaa gccctatcaa tgtaaggaat gtgggaaagc cttcagtatc aatgccaaac 1621 taactaggca tcagaggata catactgggg agaaaccttt caaatgtatg gaatgtgaga 1681 aagcattcag ctgtagttct aactataTTG TGCACCAGAG AATCCATACA GGAGAGAAAC 1741 CCTTTCAGTG TAAGGAGTGT GGAAAAGCCT TCCATGTTAA TGCCCATTTA ATTCGGCATC 1801 AGAGAAGCCA CACTGGGGAG AAACCCTTCA GATGTGTGGA ATGTGGCAAA GGCTTCAGCT 1861 TTAGTTCTGA CTACATTATA CATCAGACAG TCCACACTTG GAAGAAACCC TATATGTGTA 1921 GTGTGTGTGG GAAAGCATTC AGGTTTAGCT TCCAGCTCAG TCAGCATCAG AGTGTCCATA 1981 GTGAAGGAAA ATCCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2041 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2101 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACGCATAGGC GATTCACTTT 2161 ACGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt