Construct: ORF TRCN0000467022
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009186.1_s317c1
- Derived from:
- ccsbBroadEn_11947
- DNA Barcode:
- TGCTTCTGCTGGGTACGCTTGCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SIDT2 (51092)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467022
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51092 | SIDT2 | SID1 transmembrane family m... | NM_001040455.2 | 98.6% | 98.5% | 532T>C;1014_1015insGCTTCTCTCCTT;1159_1179del |
| 2 | mouse | 214597 | Sidt2 | SID1 transmembrane family, ... | XM_006510147.2 | 89.2% | 94.8% | (many diffs) |
| 3 | mouse | 214597 | Sidt2 | SID1 transmembrane family, ... | NM_172257.4 | 88.8% | 94.3% | (many diffs) |
| 4 | mouse | 214597 | Sidt2 | SID1 transmembrane family, ... | XM_006510145.3 | 87.2% | 92.2% | (many diffs) |
| 5 | mouse | 214597 | Sidt2 | SID1 transmembrane family, ... | XM_006510144.2 | 87% | 92.5% | (many diffs) |
| 6 | mouse | 214597 | Sidt2 | SID1 transmembrane family, ... | NM_001289668.1 | 86.6% | 92% | (many diffs) |
| 7 | mouse | 214597 | Sidt2 | SID1 transmembrane family, ... | XM_006510143.3 | 85.1% | 90% | (many diffs) |
| 8 | mouse | 214597 | Sidt2 | SID1 transmembrane family, ... | XM_006510142.2 | 82.8% | 87.6% | (many diffs) |
| 9 | mouse | 214597 | Sidt2 | SID1 transmembrane family, ... | XM_006510141.1 | 80.9% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2556
- ORF length:
- 2487
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttcgctctg ggcttgccct tcttggtgct cttggtggcc tcggtcgaga 121 gccatctggg ggttctgggg cccaagaacg tctcgcagaa agacgccgag tttgagcgca 181 cctacgtgga cgaggtcaac agcgagctgg tcaacatcta caccttcaac catactgtga 241 cccgcaacag gacagagggc gtgcgtgtgt ctgtgaacgt cctgaacaag cagaaggggg 301 cgccgttgct gtttgtggtc cgccagaagg aggctgtggt gtccttccag gtgcccctaa 361 tcctgcgagg gatgtttcag cgcaagtacc tctaccaaaa agtggaacga accctgtgtc 421 agccccccac caagaatgag tcggagattc agttcttcta cgtggatgtg tccaccctgt 481 caccagtcaa caccacatac cagctccggg tcagccgcat ggacgatttt gtgctcagga 541 ctggggagca gttcagcttc aataccacag cagcacagcc ccagtacttc aagtatgagc 601 tccctgaagg cgtggactcg gtaattgtca aggtgacctc caacaaggcc ttcccctgct 661 cagtcatctc cattcaggat gtgctgtgtc ctgtctatga cctggacaac aacgtagcct 721 tcatcggcat gtaccagacg atgaccaaga aggcggccat caccgtacag cgcaaagact 781 tccccagcaa cagcttttat gtggtggtgg tggtgaagac cgaagaccaa gcctgcgggg 841 gctccctgcc tttctacccc ttcgcagaag atgaaccggt cgatcaaggg caccgccaga 901 aaaccctgtc agtgctggtg tctcaagcag tcacgtctga ggcatacgtc agtgggatgc 961 tcttttgcct gggtatattt ctctcctttt acctgctgac cgtcctcctg gcctgctggg 1021 agaactggag gcagaagaag aagaccctgc tggtggccat tgaccgagcc tgcccagaaa 1081 gcgcttctct ccttggtcac cctcgagtcc tggctgattc ttttcctggc agttcccctt 1141 atgagggtta caactatggc tcctttgaga atgtttctgg atctaccgat ggtctggttg 1201 acagcgctgg cactggggac ctctcttacg gttaccaggg tactcggccc cgagtggact 1261 ccatgagctc tgtggaggag gatgactacg acacattgac cgacatcgat tccgacaaga 1321 atgtcattcg caccaagcaa tacctctatg tggctgacct ggcacggaag gacaagcgtg 1381 ttctgcggaa aaagtaccag atctacttct ggaacattgc caccattgct gtcttctatg 1441 cccttcctgt ggtgcagctg gtgatcacct accagacggt ggtgaatgtc acagggaatc 1501 aggacatctg ctactacaac ttcctctgcg cccacccact gggcaatctc agcgccttca 1561 acaacatcct cagcaacctg gggtacatcc tgctggggct gcttttcctg ctcatcatcc 1621 tgcaacggga gatcaaccac aaccgggccc tgctgcgcaa tgacctctgt gccctggaat 1681 gtgggatccc caaacacttt gggcttttct acgccatggg cacagccctg atgatggagg 1741 ggctgctcag tgcttgctat catgtgtgcc ccaactatac caatttccag tttgacacat 1801 cgttcatgta catgatcgcc ggactctgca tgctgaagct ctaccagaag cggcacccgg 1861 acatcaacgc cagcgcctac agtgcctacg cctgcctggc cattgtcatc ttcttctctg 1921 tgctgggcgt ggtctttggc aaagggaaca cggcgttctg gatcgtcttc tccatcattc 1981 acatcatcgc caccctgctc ctcagcacgc agctctatta catgggccgg tggaaactgg 2041 actcggggat cttccgccgc atcctccacg tgctctacac agactgcatc cggcagtgca 2101 gcgggccgct ctacgtggac cgcatggtgc tgctggtcat gggcaacgtc atcaactggt 2161 cgctggctgc ctatgggctt atcatgcgcc ccaatgattt cgcttcctac ttgttggcca 2221 ttggcatctg caacctgctc ctttacttcg ccttctacat catcatgaag ctccggagtg 2281 gggagaggat caagctcatc cccctgctcT GCATCGTTTG CACCTCCGTG GTCTGGGGCT 2341 TCGCGCTCTT CTTCTTCTTC CAGGGACTCA GCACCTGGCA GAAAACCCCT GCAGAGTCGA 2401 GGGAGCACAA CCGGGACTGC ATCCTCCTCG ACTTCTTTGA CGACCACGAC ATCTGGCACT 2461 TCCTCTCCTC CATCGCCATG TTCGGGTCCT TCCTGGTGTT GCTGACACTG GATGACGACC 2521 TGGATACTGT GCAGCGGGAC AAGATCTATG TCTTCTTGCC AACTTTCTTG TACAAAGTGG 2581 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2641 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2701 ATGCTTCTGC TGGGTACGCT TGCTAACGCG TTAAGTCgac aatcaacctc tggattacaa 2761 aatttgtgaa agatt