Construct: ORF TRCN0000467029
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017850.1_s317c1
- Derived from:
- ccsbBroadEn_03260
- DNA Barcode:
- CCCACTCCTTGTCATCCAGAGAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PBDC1 (51260)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467029
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51260 | PBDC1 | polysaccharide biosynthesis... | NM_016500.5 | 100% | 100% | |
| 2 | human | 51260 | PBDC1 | polysaccharide biosynthesis... | NM_001300888.1 | 72.6% | 43.8% | 296_297ins112;588_696del |
| 3 | mouse | 67683 | Pbdc1 | polysaccharide biosynthesis... | NM_026312.5 | 72.9% | 72.9% | (many diffs) |
| 4 | mouse | 67683 | Pbdc1 | polysaccharide biosynthesis... | NM_001281871.1 | 49.6% | 51.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 765
- ORF length:
- 699
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccaccagt ggaactgatg agccggtttc cggggagttg gtgtctgtgg 121 cacatgcgct ttctctccca gcagagtcgt atggcaacga tcctgacatt gagatggctt 181 gggccatgag agcaatgcag catgctgaag tctattacaa gctgatttca tcagttgacc 241 cacagttcct gaaactcacc aaagtagatg accaaattta ctctgagttc cggaaaaatt 301 ttgagaccct taggatagat gtgttggacc cagaagaact caagtcagaa tcagccaaag 361 agaagtggag gcCATTCTGC TTGAAGTTTA ATGGGATTGT TGAAGACTTC AACTATGGTA 421 CTTTGCTGCG ACTAGATTGT TCTCAGGGCT ACACTGAGGA AAACACCATC TTTGCCCCCA 481 GGATACAATT CTTTGCCATT GAAATTGCTC GGAACCGGGA AGGCTATAAC AAAGCTGTTT 541 ATATCAGTGT TCAGGACAAA GAAGGAGAGA AAGGAGTCAA CAATGGAGGA GAAAAAAGAG 601 CTGACAGTGG AGAAGAAGAG AACACCAAGA ATGGAGGAGA GAAAGGAGCT GATAGTGGAG 661 AAGAAAAAGA GGAAGGAATC AACAGAGAAG ACAAAACTGA CAAAGGAGGA GAAAAAGGGA 721 AAGAAGCTGA CAAAGAAATC AACAAAAGTG GTGAAAAAGC TATGTACCCA ACTTTCTTGT 781 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 841 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 901 GAAAGGACGA CCCACTCCTT GTCATCCAGA GAGGACGCGT TAAGTCgaca atcaacctct 961 ggattacaaa atttgtgaaa gatt