Construct: ORF TRCN0000467058
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014591.1_s317c1
- Derived from:
- ccsbBroadEn_12164
- DNA Barcode:
- CCGGTGCGTTAATTACAAGCCTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HEATR1 (55127)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467058
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55127 | HEATR1 | HEAT repeat containing 1 | NM_018072.6 | 5.5% | 5.5% | 66T>C;361_6432del |
| 2 | mouse | 217995 | Heatr1 | HEAT repeat containing 1 | NM_144835.4 | 4.8% | 5.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 426
- ORF length:
- 360
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGAC GTCCTTAGCC CAGCAGCTGC AACGACTCGC CCTCCCTCAA AGTGATGCCA 121 GCCTCTTATC CAGAGATGAA GTTGCTTCTT TGTTATTTGA CCCTAAGGAA GCGGCCACAA 181 TCGACAGGGA CACCGCCTTC GCCATTGGAT GTACTGGCCT GGAAGAGTTG CTTGGAATTG 241 ATCCTTCCTT TGAGCAGTTT GAAGCACCGT TGTTCAGTCA GCTAGCAAAA ACCTTGGAGC 301 GAAGTGTTCA GACCAAAGCA GTAAACAAAC AGTTGGATGA AAACATTTCA TTATTCCTTA 361 TTCACTTGTC GCCTTACTTC CTGCTTAAGC CAGCACAGAA GTGTCTGGAG TGGTTGATTC 421 ACAGGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 481 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 541 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCGGTGCGT TAATTACAAG CCTGGACGCG 601 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt