Transcript: Mouse NM_144835.4

Mus musculus HEAT repeat containing 1 (Heatr1), mRNA.

Source:
NCBI, updated 2015-02-15
Taxon:
Mus musculus (mouse)
Gene:
Heatr1 (217995)
Length:
6776
CDS:
129..6560

Additional Resources:

NCBI RefSeq record:
NM_144835.4
NBCI Gene record:
Heatr1 (217995)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_144835.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000376857 GAAACGGTTCTCAGCTATATT pLKO_005 4779 CDS 100% 15.000 21.000 N Heatr1 n/a
2 TRCN0000366854 GGGTTCTGATTAGGCTTATAA pLKO_005 2665 CDS 100% 15.000 21.000 N Heatr1 n/a
3 TRCN0000366853 ACCCGGTTAGGCGATGATAAT pLKO_005 1698 CDS 100% 13.200 18.480 N Heatr1 n/a
4 TRCN0000376812 CCATGTTACTTTCGGTACTTT pLKO_005 6640 3UTR 100% 5.625 7.875 N Heatr1 n/a
5 TRCN0000379345 GTGAGACTTCTGGCCGTTAAT pLKO_005 1608 CDS 100% 13.200 9.240 N Heatr1 n/a
6 TRCN0000375729 TAGCCTTTCAAATCCACTTAA pLKO_005 2753 CDS 100% 13.200 9.240 N Heatr1 n/a
7 TRCN0000177013 GCAGTTAATTCTGAGTTGTTT pLKO.1 3884 CDS 100% 5.625 3.938 N Heatr1 n/a
8 TRCN0000197859 GTCACACTCATTCTCATGTTT pLKO.1 6603 3UTR 100% 5.625 3.938 N Heatr1 n/a
9 TRCN0000182279 GCCAGCCATTAGCAAGACTTT pLKO.1 5594 CDS 100% 4.950 3.465 N Heatr1 n/a
10 TRCN0000366793 TACCATGAGACACGGGTATTT pLKO_005 540 CDS 100% 0.000 0.000 N Heatr1 n/a
11 TRCN0000375679 GAAATCCTCTTTACCAGATTA pLKO_005 878 CDS 100% 13.200 7.920 N Heatr1 n/a
12 TRCN0000177211 GCTTTGCTTAATGAGCAGTTT pLKO.1 1383 CDS 100% 4.950 2.970 N Heatr1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_144835.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12163 pDONR223 100% 13.8% 15.1% None (many diffs) n/a
2 ccsbBroad304_12163 pLX_304 0% 13.8% 15.1% V5 (many diffs) n/a
3 TRCN0000473108 CCGTTACGCATCTTTGCGTAGGCT pLX_317 49.6% 13.8% 15.1% V5 (many diffs) n/a
4 ccsbBroadEn_12164 pDONR223 100% 4.8% 5.2% None (many diffs) n/a
5 ccsbBroad304_12164 pLX_304 0% 4.8% 5.2% V5 (many diffs) n/a
6 TRCN0000467058 CCGGTGCGTTAATTACAAGCCTGG pLX_317 100% 4.8% 5.2% V5 (many diffs) n/a
Download CSV