Construct: ORF TRCN0000467059
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017559.1_s317c1
- Derived from:
- ccsbBroadEn_03091
- DNA Barcode:
- CCGGAGGTCTCTCACAATCTACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMOD2 (29767)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467059
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29767 | TMOD2 | tropomodulin 2 | NM_014548.4 | 100% | 100% | |
| 2 | human | 29767 | TMOD2 | tropomodulin 2 | NM_001142885.2 | 89.7% | 89.7% | 624_625ins108 |
| 3 | human | 29767 | TMOD2 | tropomodulin 2 | XM_017022089.2 | 85.4% | 83.5% | 875_876ins145;909_918delTAAACTTCCT |
| 4 | mouse | 50876 | Tmod2 | tropomodulin 2 | NM_001038710.1 | 90% | 95.1% | (many diffs) |
| 5 | mouse | 50876 | Tmod2 | tropomodulin 2 | NM_016711.3 | 90% | 95.1% | (many diffs) |
| 6 | mouse | 50876 | Tmod2 | tropomodulin 2 | XM_006511267.3 | 90% | 95.1% | (many diffs) |
| 7 | mouse | 50876 | Tmod2 | tropomodulin 2 | XM_006511268.2 | 90% | 95.1% | (many diffs) |
| 8 | mouse | 50876 | Tmod2 | tropomodulin 2 | XM_017313459.1 | 90% | 95.1% | (many diffs) |
| 9 | mouse | 50876 | Tmod2 | tropomodulin 2 | XM_017313460.1 | 90% | 95.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1119
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc actccccttt caaaaagagc tggagaaata caagaacatt gatgaagatg 121 agcttcttgg caaactctca gaagaggaac tgaaacagtt ggaaaatgtt ctagatgacc 181 tagatcctga gagtgccatg ctgccagctg gatttcgaca gaaagaccag acacagaagg 241 cagccaccgg cccctttgac cgcgagcacc tcctcatgta cctggagaag gaggctttgg 301 aacagaaaga cagagaggac tttgtgccct tcactggaga aaagaaaggg agagtcttta 361 tccctaaaga aaagcctata gaaactcgta aagaagaaaa agtgaccctt gacccagaac 421 tggaagaagc tttggccagt gcctctgaca ccgaactcta tgatcttgca gctgtccttg 481 gagtacacaa tttgctcaac aatccaaagt tcgatgaaga aacagccaac aataaaggtg 541 gcaaaggacc tgtcagaaat gttgtcaaag gtgaaaaagt aaagccagta tttgaggaac 601 caccaaatcc cacaaatgtg gaaataagcc tgcagcagat gaaagccaat gatcctagct 661 tgcaagaagt caacctcaac aacattaaga aCATTCCAAT TCCAACCCTG AGGGAATTTG 721 CAAAGGCTCT GGAGACCAAC ACTCACGTGA AGAAGTTCAG CCTGGCCGCA ACTCGCAGCA 781 ATGACCCTGT GGCCATTGCT TTTGCAGACA TGCTGAAAGT AAACAAGACC TTGACAAGTC 841 TAAACATAGA ATCCAATTTT ATCACTGGAA CTGGGATCCT GGCCCTGGTA GAGGCACTGA 901 AAGAAAATGA CACCTTGACA GAAATCAAGA TTGACAACCA GAGGCAGCAG TTGGGAACAG 961 CTGTAGAGAT GGAAATTGCC CAGATGCTGG AGGAGAATTC AAGGATCCTC AAGTTTGGAT 1021 ACCAGTTTAC CAAGCAAGGG CCACGAACAA GGGTGGCAGC TGCCATCACA AAGAATAATG 1081 ACCTGGTTCG TAAGAAGAGA GTTGAAGCAG ACCGAAGGTA CCCAACTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGACCGGAG GTCTCTCACA ATCTACTAAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt