Construct: ORF TRCN0000467081
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014596.1_s317c1
- Derived from:
- ccsbBroadEn_01358
- DNA Barcode:
- AATGGGAAATGACATTGAAAATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB3A (5864)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467081
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5864 | RAB3A | RAB3A, member RAS oncogene ... | NM_002866.5 | 100% | 100% | |
| 2 | human | 5864 | RAB3A | RAB3A, member RAS oncogene ... | XM_011528164.2 | 100% | 100% | |
| 3 | mouse | 19339 | Rab3a | RAB3A, member RAS oncogene ... | NM_001166399.2 | 89.2% | 99% | (many diffs) |
| 4 | mouse | 19339 | Rab3a | RAB3A, member RAS oncogene ... | NM_001328047.1 | 89.2% | 99% | (many diffs) |
| 5 | mouse | 19339 | Rab3a | RAB3A, member RAS oncogene ... | NM_009001.6 | 89.2% | 99% | (many diffs) |
| 6 | mouse | 19339 | Rab3a | RAB3A, member RAS oncogene ... | XM_006509603.3 | 89.2% | 99% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 726
- ORF length:
- 660
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc atccgccaca gactcgcgct atgggcagaa ggagtcctcg gatcagaact 121 tcgactacat gttcaagatt ctcatcatcg gcaacagcag cgtgggcaag acgtccttcc 181 tcttccgcta tgctgacgac tcgttcacgc ctgccttcgt cagcaccgtg ggcatcgact 241 tcaaggtcaa gaccatctat cgcaacgaca agaggatcaa gctgcagatc tgggacacag 301 cagggcaaga gcggtaccgg accatcacca ccgcatacta ccggGGCGCT ATGGGCTTCA 361 TCCTCATGTA TGACATCACC AACGAGGAAT CCTTCAATGC AGTGCAGGAC TGGTCCACCC 421 AGATCAAGAC CTACTCATGG GACAATGCCC AGGTGCTGCT GGTAGGAAAC AAGTGTGACA 481 TGGAGGATGA GCGGGTGGTG TCATCAGAAC GTGGCCGGCA GCTAGCTGAC CACCTTGGGT 541 TCGAGTTCTT TGAGGCAAGC GCCAAGGACA ACATTAACGT CAAGCAGACC TTTGAGCGCC 601 TGGTGGATGT CATCTGCGAG AAGATGTCCG AGTCGTTGGA CACGGCGGAC CCTGCGGTCA 661 CAGGCGCCAA GCAGGGCCCA CAGCTCAGTG ACCAGCAGGT GCCACCGCAC CAGGACTGCG 721 CCTGCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 781 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 841 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAATGGGAAA TGACATTGAA AATTAACGCG 901 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt