Construct: ORF TRCN0000467095
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016145.1_s317c1
- Derived from:
- ccsbBroadEn_06535
- DNA Barcode:
- CCCTTGATCCAGACTGAGTATTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LPL (4023)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467095
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4023 | LPL | lipoprotein lipase | NM_000237.3 | 99.9% | 99.7% | 953A>G |
| 2 | mouse | 16956 | Lpl | lipoprotein lipase | NM_008509.2 | 88% | 92.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1491
- ORF length:
- 1425
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gagcaaagcc ctgctcgtgc tgactctggc cgtgtggctc cagagtctga 121 ccgcctcccg cggaggggtg gccgccgccg accaaagaag agattttatc gacatcgaaa 181 gtaaatttgc cctaaggacc cctgaagaca cagctgagga cacttgccac ctcattcccg 241 gagtagcaga gtccgtggct acctgtcatt tcaatcacag cagcaaaacc ttcatggtga 301 tccatggctg gacggtaaca ggaatgtatg agagttgggt gccaaaactt gtggccgccc 361 tgtacaagag agaaccagac tccaatgtca ttgtggtgga ctggctgtca cgggctcagg 421 agcattaccc agtgtccgcg ggctacacca aactggtggg acaggatgtg gcccggttta 481 tcaactggat ggaggaggag tttaactacc ctctggacaa tgtccatctc ttgggataca 541 gccttggagc ccatgctgct ggcattgcag gaagtctgac caataagaaa gtcaacagaa 601 ttactggcct cgatccagct ggacctaact ttgagtatgc agaagccccg agtcgtcttt 661 ctcctgatga tgcagatttt gtagacgtct tacacacatt caccagaggg tcccctggtc 721 gaagcattgg aatccagaaa ccagttgggc atgttgacat ttacccgaat ggaggtactt 781 ttcagccagg atgtaacatt ggagaagcta tccgcgtgat tgcagagaga ggacttggag 841 atgtggacca gctagtgaag tgctcccacg agcgctccat tcatctcttc atcgactctc 901 tgttgaatga agaaaatcca agtaaggcct acaggtgcag ttccaaggaa gcctttgaga 961 aagggctctg cttgagttgt agaaagaacc gctgcaacaa tctgggctat gagatcagta 1021 aagtcagagc caaaagaagc agcaaaatgt acctgaagac tcgttcTCAG ATGCCCTACA 1081 AAGTCTTCCA TTACCAAGTA AAGATTCATT TTTCTGGGAC TGAGAGTGAA ACCCATACCA 1141 ATCAGGCCTT TGAGATTTCT CTGTATGGCA CCGTGGCCGA GAGTGAGAAC ATCCCATTCA 1201 CTCTGCCTGA AGTTTCCACA AATAAGACCT ACTCCTTCCT AATTTACACA GAGGTAGATA 1261 TTGGAGAACT ACTCATGTTG AAGCTCAAAT GGAAGAGTGA TTCATACTTT AGCTGGTCAG 1321 ACTGGTGGAG CAGTCCCGGC TTCGCCATTC AGAAGATCAG AGTAAAAGCA GGAGAGACTC 1381 AGAAAAAGGT GATCTTCTGT TCTAGGGAGA AAGTGTCTCA TTTGCAGAAA GGAAAGGCAC 1441 CTGCGGTATT TGTGAAATGC CATGACAAGT CTCTGAATAA GAAGTCAGGC TGCCCAACTT 1501 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1561 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1621 CTTGTGGAAA GGACGACCCT TGATCCAGAC TGAGTATTTA ACGCGTTAAG TCgacaatca 1681 acctctggat tacaaaattt gtgaaagatt