Construct: ORF TRCN0000467107
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016655.1_s317c1
- Derived from:
- ccsbBroadEn_02536
- DNA Barcode:
- GTTATGTTGGCCATTTATTACTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPLX1 (10815)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467107
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10815 | CPLX1 | complexin 1 | NM_006651.4 | 100% | 100% | |
| 2 | human | 10815 | CPLX1 | complexin 1 | XM_011513391.1 | 88.8% | 88.8% | 0_1ins45 |
| 3 | mouse | 12889 | Cplx1 | complexin 1 | NM_007756.3 | 87% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 468
- ORF length:
- 402
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtttgtgatg aagcaggctc taggaggggc caccaaggac atggggaaga 121 tgctgggggg tgacgaggag aaggacccag acgccgccaa gaaggaggag gagcggcagg 181 aggcgctgcg ccaggcggag gaggagcgca aggccaagta cgccaagatg gaggcggagc 241 gcgaggccgt gcgccagggc atccgagaca agtacggcat CAAGAAGAAG GAGGAGCGCG 301 AGGCCGAGGC CCAGGCCGCC ATGGAGGCCA ACTCCGAGGG GAGCTTGACG CGGCCCAAGA 361 AGGCCATCCC GCCGGGCTGC GGGGACGAGG TGGAGGAGGA GGACGAGAGC ATCCTGGACA 421 CCGTCATCAA GTACCTGCCC GGGCCGCTGC AGGACATGCT CAAGAAGTAC CCAACTTTCT 481 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 541 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 601 GTGGAAAGGA CGAGTTATGT TGGCCATTTA TTACTCAACG CGTTAAGTCg acaatcaacc 661 tctggattac aaaatttgtg aaagatt