Construct: ORF TRCN0000467129
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005284.1_s317c1
- Derived from:
- ccsbBroadEn_09335
- DNA Barcode:
- AATGAGAAATCGCCCGGTTAGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM129 (92305)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467129
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92305 | TMEM129 | transmembrane protein 129 | NM_138385.3 | 99.7% | 99.5% | 213T>C;686G>C |
| 2 | human | 92305 | TMEM129 | transmembrane protein 129 | NM_001127266.2 | 63.6% | 62.7% | (many diffs) |
| 3 | human | 92305 | TMEM129 | transmembrane protein 129 | XM_005248038.4 | 43.5% | 42.5% | (many diffs) |
| 4 | human | 92305 | TMEM129 | transmembrane protein 129 | XM_005248039.5 | 43.5% | 42.5% | (many diffs) |
| 5 | human | 92305 | TMEM129 | transmembrane protein 129 | XM_011513594.3 | 43.5% | 42.5% | (many diffs) |
| 6 | human | 92305 | TMEM129 | transmembrane protein 129 | XM_017008834.1 | 43.5% | 42.5% | (many diffs) |
| 7 | mouse | 68366 | Tmem129 | transmembrane protein 129 | NM_026698.2 | 54.1% | 55.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 763
- ORF length:
- 696
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcnatgg acagccccga ggtgaccttc actctcgcct atctggtgtt cgccgtgtgc 121 ttcgtgttca cgcccaacga gttccacgcg gcggggctca cggtgcagaa cctgctgtcg 181 ggctggctgg gcagcgagga cgccgccttc gtgcccttcc acttgcgccg cacggccgcc 241 acgctgttgt gccactcgct gctgccgctc ggctactacg tgggcatgtg ccttgcggct 301 tcagaaaagc ggctccacgc cctcagccag gccccTGAGG CCTGGCGGCT CTTCCTGCTG 361 CTGGCCGTGA CCCTCCCCTC CATCGCCTGC ATCCTGATCT ACTACTGGTC CCGTGACCGG 421 TGGGCCTGCC ACCCACTGGC GCGCACCCTG GCCCTCTACG CCCTCCCACA GTCTGGCTGG 481 CAGGCTGTTG CCTCCTCTGT CAACACTGAG TTCCGGCGGA TTGACAAGTT TGCCACCGGT 541 GCACCAGGTG CCCGTGTGAT TGTGACAGAC ACGTGGGTGA TGAAGGTAAC CACCTACCGA 601 GTGCACGTGG CCCAGCAGCA GGACGTGCAC CTGACTGTGA CGGAGTCTCG GCAGCATGAG 661 CTCTCGCCAG ACTCGAACTT GCCCGTGCAG CTCCTCACCA TCCGTGTGGC CAGCACCAAC 721 CCTGCTGTGC AGGCCTTTGA CATCTGGAGC TCGAGGCCTG CATACCCAAC TTTCTTGTAC 781 AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG 841 TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA 901 AAGGACGAAA TGAGAAATCG CCCGGTTAGT GCACGCGTTA AGTCgacaat caacctctgg 961 attacaaaat ttgtgaaaga tt