Construct: ORF TRCN0000467143
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013839.1_s317c1
- Derived from:
- ccsbBroadEn_08614
- DNA Barcode:
- CTGAGTATGAATGCTAATCGCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFA12 (55967)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467143
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55967 | NDUFA12 | NADH:ubiquinone oxidoreduct... | NM_018838.5 | 99.7% | 99.3% | 310A>G |
| 2 | human | 55967 | NDUFA12 | NADH:ubiquinone oxidoreduct... | NM_001258338.1 | 43.4% | 40% | 167_168ins88;189_190ins158 |
| 3 | mouse | 66414 | Ndufa12 | NADH dehydrogenase (ubiquin... | NM_025551.3 | 83.4% | 85.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 501
- ORF length:
- 435
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gttagtgcag gtccTGAAAC GCGGGCTGCA GCAGATCACC GGCCACGGCG 121 GTCTCCGAGG CTATCTACGG GTTTTTTTCA GGACAAATGA TGCGAAGGTT GGTACATTAG 181 TGGGGGAAGA CAAATATGGA AACAAATACT ATGAAGACAA CAAGCAATTT TTTGGCCGTC 241 ACCGATGGGT TGTATATACT ACTGAAATGA ATGGCAAAAA CACATTCTGG GATGTGGATG 301 GAAGCATGGT GCCTCCTGAA TGGCATCGTT GGCTTCACAG TATGACTGAT GATCCTCCAA 361 CAACAAAACC ACTTGCTGCT CGTAAATTCA TTTGGACGAA CCATAAATTC AACGTGACTG 421 GCACCCCAGA ACAATATGTA CCTTATTCTA CCACTAGAAA GAAGATTCAG GAGTGGATCC 481 CACCTTCAAC ACCTTACAAG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTGA GTATGAATGC 661 TAATCGCCCT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt