Construct: ORF TRCN0000467150
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014088.1_s317c1
- Derived from:
- ccsbBroadEn_07897
- DNA Barcode:
- GCTTCATAGTAAGAAGGTACGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V0A2 (23545)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467150
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23545 | ATP6V0A2 | ATPase H+ transporting V0 s... | NM_012463.4 | 99.8% | 100% | 426T>C;471T>C;1515T>C |
| 2 | human | 23545 | ATP6V0A2 | ATPase H+ transporting V0 s... | XM_024448910.1 | 95.2% | 95.3% | (many diffs) |
| 3 | human | 23545 | ATP6V0A2 | ATPase H+ transporting V0 s... | XM_024448911.1 | 79.8% | 79.7% | (many diffs) |
| 4 | human | 23545 | ATP6V0A2 | ATPase H+ transporting V0 s... | XM_024448912.1 | 67.8% | 67.8% | 0_1ins822;2_3delTGinsCT;693T>C |
| 5 | mouse | 21871 | Atp6v0a2 | ATPase, H+ transporting, ly... | NM_011596.5 | 84.2% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2634
- ORF length:
- 2568
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gtccctgttc cggagcgaga ccatgtgcct ggcgcagctc ttcctgcagt 121 cgggcacggc ctacgagtgc ctcagcgccc tgggcgagaa aggcctggtc cagttccgag 181 acctcaacca gaacgtaagt tctttccaaa gaaaatttgt tggtgaggtg aagaggtgtg 241 aagagctaga gcgaatattg gtgtatttgg tacaggaaat taatagagct gatattcccc 301 ttcctgaagg agaggccagc cctcctgcgc cacccctgaa acaggttcta gaaatgcagg 361 agcagttgca gaagctcgag gttgaactga gagaagtcac taagaacaag gagaaactga 421 ggaaaaactt gctggaactg atagagtaca ctcacatgct gagagtgaca aagacctttg 481 tgaaacgcaa cgttgagttt gaacccactt atgaagaatt cccttcctta gagagcgatt 541 ctttgttgga ttacagctgt atgcagaggc tgggagcaaa actgggattt gtgtctggcc 601 taattaacca aggaaaagtg gaagcatttg aaaaaatgtt gtggagagtc tgcaaagggt 661 acaccatcgt gtcctatgca gaactggatg aatcccttga agaccctgaa acaggggaag 721 tcataaaatg gtatgtcttt ttaatatcct tttggggaga gcagattggc cacaaggtta 781 agaagatatg tgattgctac cactgccacg tgtaccccta tccaaacaca gccgaggagc 841 ggagggagat ccaggagggg ctgaacaccc gcatccagga tctctacact gtactgcaca 901 aaaccgagga ctatttgagg caagtgctat gtaaagccgc cgagtctgtc tacagccgtg 961 tgatccaggt gaagaaaatg aaggccatct atcacatgct gaacatgtgc agctttgacg 1021 tgaccaacaa gtgcctcatt gctgaggtct ggtgtcccga ggcggatctg caggacctgc 1081 gccgggcact ggaggagggc tcgagagaga gtggtgctac aatcccctca ttcatgaata 1141 taatccccac aaaagaaaca ccccccactc ggatccgcac caacaaattc accgagggat 1201 ttcagaacat cgtggatgct tatggagtcg gaagctacag agaagtcaat ccagctctct 1261 ttaccatcat caccttcccg tttttatttg ctgtgatgtt tggagacttc ggacatggct 1321 ttgtgatgtt tttatttgcc ctcttgttgg tgttaaatga aaatcatccc agactaaatc 1381 agtcacaaga gatcatgagg atgtttttta atggccggta catcctcctg ctgatggggc 1441 tgttctcagt gtacactggc ctcatctaca acgactgctt ttcaaagtca gtcaacctgt 1501 tcggctctgg gtggaacgtg tcggccatgt acagctccag ccacccaccc gcagagcata 1561 agaagatggt gctttggaac gacagcgtcg ttagacacaa cagcattttg cagctggatc 1621 caagcattcc tggagtgttc cgaggccctt atccccttgg cattgatcct atttggaact 1681 tggccacaaa tcgcctcact tttctaaact ctttcaaaat gaaaatgtcc gtgattttag 1741 gaatcattca tatgactttt ggagtcattc tgggaatatt taaccacttg cacttcagga 1801 agaagttcaa catttacctg gtttccatcc cggaacttct cttcatgctc tgtatctttg 1861 gataccttat atttatgatt ttctacaagt ggctggtttt ttcagcagaa acctccagag 1921 ttgctcccag cattctgatt gaatttatta acatgttttt attcccagcc agtaaaacaa 1981 gtggccttta cacagggcag gagtatgtcc agagagtgct gctggttgtc acagcattgt 2041 ctgtccctgt cctcttcttg ggaaagccac tgtttttgtt gtggcttcac aatgggcgta 2101 gttgcttcgg ggtgaaccgg agtggctaca cacttataag gaaagatagt gaggaagaag 2161 tttcattgct gggaagccaa gatatagaag agggaaatca ccaggtggaa gatggatgta 2221 gagaaatggc gtgtgaagag tttaattttg gagaaatatt aatgacccaa gtaatccatt 2281 ccatcgagta ctgtctggga tgcatctcca acaccgcctc ctaccTGAGG CTCTGGGCGC 2341 TTAGCCTGGC TCACGCACAG TTGTCTGATG TCCTGTGGGC CATGCTGATG CGCGTGGGCC 2401 TCCGCGTTGA CACCACCTAT GGCGTCTTGC TACTGCTCCC GGTTATCGCG CTCTTTGCAG 2461 TTTTGACCAT TTTCATCCTT CTGATCATGG AAGGGCTTTC TGCGTTTCTT CACGCCATAC 2521 GCCTCCACTG GGTAGAATTT CAGAACAAAT TCTACGTTGG TGCAGGCACC AAATTTGTTC 2581 CTTTCTCATT CAGTCTACTT TCATCAAAGT TCAATAACGA CGACAGTGTG GCATGCCCAA 2641 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2701 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2761 TATCTTGTGG AAAGGACGAG CTTCATAGTA AGAAGGTACG TCAACGCGTT AAGTCgacaa 2821 tcaacctctg gattacaaaa tttgtgaaag att