Construct: ORF TRCN0000467156
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012171.1_s317c1
- Derived from:
- ccsbBroadEn_07040
- DNA Barcode:
- ACGTAATGGAGAAAGATACAATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MLX (6945)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467156
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6945 | MLX | MAX dimerization protein MLX | NM_198204.2 | 99.8% | 100% | 639G>A |
| 2 | human | 6945 | MLX | MAX dimerization protein MLX | NM_198205.1 | 87.5% | 87.7% | 78_79ins90;549G>A |
| 3 | human | 6945 | MLX | MAX dimerization protein MLX | NM_170607.2 | 81.7% | 81.8% | 39_200del;801G>A |
| 4 | mouse | 21428 | Mlx | MAX-like protein X | NM_001159385.1 | 90.9% | 98.3% | (many diffs) |
| 5 | mouse | 21428 | Mlx | MAX-like protein X | NM_001159384.1 | 78.9% | 86.4% | (many diffs) |
| 6 | mouse | 21428 | Mlx | MAX-like protein X | NM_011550.3 | 74.4% | 80.5% | (many diffs) |
| 7 | mouse | 21428 | Mlx | MAX-like protein X | XM_006532809.3 | 73.8% | 70.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 798
- ORF length:
- 732
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ggagccgggc gcctctcccg aggacccttg ggtcaaggtg gagtatgcct 121 acagcgacaa cagcctggac cccgggcttt ttgtagaaag cacccgcaag gggagtgtag 181 tgtccagagc taatagcatc ggttccacca gtgcctcttc tgtccccaac acagatgatg 241 aggacagtga ttaccaccag gaggcctaca aggagtccta caaagaccgg cggcggcgcg 301 cacacactca ggctgagcag aagaggaggg acgccatcaa gagaggctat gatgaccttc 361 agaccatcgt ccccacttgc cagcagcagg acttctccat tggctcccaa aagctcagca 421 aagccatcgt tctacaaaag acCATTGACT ACATTCAGTT TTTGCACAAG GAGAAGAAAA 481 AGCAGGAGGA GGAGGTGTCC ACGTTACGCA AGGATGTCAC CGCCCTAAAG ATCATGAAAG 541 TGAACTATGA GCAGATTGTG AAGGCACACC AGGACAACCC CCATGAAGGG GAGGACCAGG 601 TCTCTGACCA GGTCAAGTTC AACGTGTTTC AAGGCATCAT GGATTCCCTG TTCCAGTCCT 661 TCAATGCCTC CATCTCAGTG GCCAGCTTCC AGGAGCTGTC AGCATGTGTC TTCAGCTGGA 721 TCGAGGAGCA CTGTAAGCCT CAGACCCTGC GGGAGATTGT GATTGGCGTC CTGCACCAAT 781 TGAAAAACCA GCTTTACTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 841 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 901 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACGTAAT GGAGAAAGAT 961 ACAATCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt