Construct: ORF TRCN0000467161
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009651.1_s317c1
- Derived from:
- ccsbBroadEn_07686
- DNA Barcode:
- CTCAACGGAAAATATCTGAGTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FRS3 (10817)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467161
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10817 | FRS3 | fibroblast growth factor re... | NM_006653.5 | 99.7% | 99.5% | 8_9delGCinsTT;1459A>T |
| 2 | human | 10817 | FRS3 | fibroblast growth factor re... | XM_011514254.2 | 99.7% | 99.5% | 8_9delGCinsTT;1459A>T |
| 3 | human | 10817 | FRS3 | fibroblast growth factor re... | XM_011514255.2 | 99.7% | 99.5% | 8_9delGCinsTT;1459A>T |
| 4 | human | 10817 | FRS3 | fibroblast growth factor re... | XM_017010193.1 | 92.4% | 92.2% | 0_1ins111;1348A>T |
| 5 | mouse | 107971 | Frs3 | fibroblast growth factor re... | NM_144939.2 | 83% | 84.8% | (many diffs) |
| 6 | mouse | 107971 | Frs3 | fibroblast growth factor re... | XM_006523468.1 | 83% | 84.8% | (many diffs) |
| 7 | mouse | 107971 | Frs3 | fibroblast growth factor re... | XM_006523469.3 | 83% | 84.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1542
- ORF length:
- 1476
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gatttgctgc agctgcctga acagagacag cgttccagac aaccacccca 121 ccaagttcaa ggtgacaaat gtggatgatg agggggtgga gctgggctct ggggtgatgg 181 agctgacgca gagtgagctg gtgctgcacc tgcatcggcg tgaggccgtc cgctggcctt 241 atctctgctt gcggcgctat ggctacgact ccaacctctt ctcctttgag agtggccgcc 301 gatgtcagac aggccaggga atatttgcat ttaagtgttc ccgggctgag gaaatcttca 361 acctccttca ggatctgatg cagtgcaaca gcatcaatgt gatggaagag cctgtcatca 421 tcacccgcaa tagccacccc gctgagcttg acctccctcg agccccccag ccacccaatg 481 ctctaggcta cactgtctcc agcttttcca atggctgccc tggagagggc ccacgattct 541 cagctccccg gcggctctcg acaagcagcc tgcggcaccc ctcgcttggg gaagagtcca 601 cccatgccct cattgctcct gatgagcagt cccacaccta tgtcaacaca ccggccagtg 661 aagatgacca ccgcaggggc cgccactgcc tgcagcccct gcctgagggt caggcaccct 721 tcctcccgca ggcccgggga cctgaccaac gggacccaca ggtgttcttg cagccaggcc 781 aggtgaagtt tgtgttgggc ccgacccctg ctcggcggca catggtgaag tgccagggcc 841 tctgtcccag cctgcatgac cccccacacc acaataataa caatgaggcc ccttctgagt 901 gtccagccca gcccaagtgc acctacgaga acgtcaccgg ggggctgtgg cgaggggctg 961 gctggagact gagcccagag gagccgggct ggaatggcct tgcccaccgc cgggccgccc 1021 tgctgcacta tgagaacctg cccccactgc cccctgtgtg ggaaagccaa gcccagcagc 1081 tgggagggga ggctggggat gatggggact cgagGGATGG GCTCACACCC TCTTCCAATG 1141 GCTTCCCTGA TGGTGAGGAG GACGAGACCC CACTGCAGAA GCCCACCAGC ACCCGGGCCG 1201 CCATCCGCAG CCACGGCAGC TTTCCTGTGC CACTGACCCG CCGCCGCGGC TCCCCAAGGG 1261 TCTTCAACTT TGATTTCCGC CGGCCGGGGC CCGAGCCCCC AAGGCAGCTT AACTACATCC 1321 AGGTGGAGCT AAAGGGCTGG GGTGGAGACC GCCCTAAGGG GCCCCAGAAC CCCTCGAGCC 1381 CCCAAGCCCC CATGCCCACC ACCCACCCTG CCCGAAGCTC AGACTCCTAC GCCGTGATTG 1441 ACCTCAAAAA GACCGTGGCC ATGTCCAACC TGCAGAGAGC TCTGCCCCGA GACGATGGCA 1501 CCGCCAGGAA AACCCGGCAC AACTGCACCG ACCTGCCTCT GTACCCAACT TTCTTGTACA 1561 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1621 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1681 AGGACGACTC AACGGAAAAT ATCTGAGTTT CACGCGTTAA GTCgacaatc aacctctgga 1741 ttacaaaatt tgtgaaagat t