Construct: ORF TRCN0000467163
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005281.1_s317c1
- Derived from:
- ccsbBroadEn_09134
- DNA Barcode:
- TCTCTTAGGATATTAATGTATTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPRTN (83932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467163
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83932 | SPRTN | SprT-like N-terminal domain | NM_032018.7 | 99.9% | 99.7% | 887C>T |
| 2 | human | 83932 | SPRTN | SprT-like N-terminal domain | XM_006711818.3 | 91.1% | 91% | 320_321ins129;758C>T |
| 3 | human | 83932 | SPRTN | SprT-like N-terminal domain | XM_011544289.2 | 69.6% | 70.1% | (many diffs) |
| 4 | human | 83932 | SPRTN | SprT-like N-terminal domain | NM_001010984.4 | 50.6% | 49% | (many diffs) |
| 5 | human | 83932 | SPRTN | SprT-like N-terminal domain | NM_001261462.3 | 41.8% | 40.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1533
- ORF length:
- 1467
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgatgacttg atgttggcac tgcggcttca ggaggagtgg aacttgcagg 121 aggcggagcg cgatcatgcc caggagtccc tgtcgctagt ggacgcgtcg tgggagttgg 181 tggaccccac accggacttg caggcactgt ttgttcagtt taacgaccaa ttcttctggg 241 gccagctgga ggccgtcgag gtgaagtgga gcgtgcgaat gaccctgtgt gctgggatat 301 gcagctatga agggaagggt ggaatgtgtt ccatccgtct cagcgaaccc cttttgaagt 361 tgaggccaag aaaggatctt gtagagaccc tcctgcatga aatgatacat gcctatttat 421 ttgtcactaa taacgacaaa gaccgagaag ggcatggtcc agaattttgt aaacatatgc 481 atcgcatcaa cagcctgact ggagccaata taacggtata ccatactttt cacgatgagg 541 tggatgagta tcggcgacac tggtggcgct gcaatgggcc gtgccagcac aggccaccgt 601 attacggcta tgtcaaacga gctactaaca gggaaccctc tgctcatgac tattggtggg 661 ctgagcacca gaaaacctgt ggaggcactt acataaaaat caaggaacca gagaattact 721 caaaaaaagg caaaggaaag gcaaaactag gaaaggaacc agtattggcc gcagagaata 781 aagataaacc caacagaggt gaggcccagc tagtaatccc ttttagtggg aaaggatatg 841 ttctaggaga aacaagcaat ttaccttcac ctgggaaact gatcacttca catgccatta 901 ataaaaccca agatctttta aatcaaaacc attcagcaaa tgctgtaaga cttaattcta 961 aaatcaaggt gaaatttgaa cagaatggtt caagtaaaaa ttctcatctg gtctcccctg 1021 ctgttagtaa cagtcaccaa aatgttctaa gcaactactt tcctagagta tcatttgcca 1081 accaaaaggc tttcagaggt gtgaatggat ctccaaggat aagtgtaaca gttggcaaca 1141 TCCCTAAAAA CTCAGTCTCT TCTAGTTCTC AGAGAAGGGT TTCATCTTCT AAGATATCCC 1201 TAAGAAATTC TTCAAAAGTA ACGGAATCAG CATCTGTGAT GCCATCCCAG GATGTGAGTG 1261 GGTCTGAAGA TACATTCCCA AATAAACGAC CTAGGCTAGA AGATAAGACT GTTTTTGACA 1321 ATTTTTTTAT CAAGAAAGAG CAAATAAAAA GCAGTGGTAA TGATCCAAAG TATAGTACAA 1381 CCACAGCTCA GAATTCCAGC AGTTCATCCA GTCAGAGCAA AATGGTTAAT TGCCCAGTTT 1441 GTCAGAATGA AGTTCTGGAG TCTCAGATTA ATGAGCACTT GGACTGGTGC CTTGAAGGTG 1501 ACAGCATCAA AGTCAAAAGC GAAGAAAGTC TTTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1561 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1621 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 1681 TCTTAGGATA TTAATGTATT TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1741 ttgtgaaaga tt