Transcript: Human XM_006711818.3

PREDICTED: Homo sapiens SprT-like N-terminal domain (SPRTN), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SPRTN (83932)
Length:
3407
CDS:
405..1745

Additional Resources:

NCBI RefSeq record:
XM_006711818.3
NBCI Gene record:
SPRTN (83932)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006711818.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000435272 GTTCTGGAGTCTCAGATTAAT pLKO_005 1662 CDS 100% 15.000 21.000 N SPRTN n/a
2 TRCN0000073312 GATCACTTCACATGCCATTAA pLKO.1 1091 CDS 100% 13.200 18.480 N SPRTN n/a
3 TRCN0000073309 GCCGCAGAGAATAAAGATAAA pLKO.1 978 CDS 100% 13.200 18.480 N SPRTN n/a
4 TRCN0000073310 CTATGTCAAACGAGCTACTAA pLKO.1 818 CDS 100% 5.625 7.875 N SPRTN n/a
5 TRCN0000434833 TACTTTCCTAGAGTATCATTT pLKO_005 1266 CDS 100% 13.200 10.560 N SPRTN n/a
6 TRCN0000414781 CATTCCCTGAACTGATGTAAA pLKO_005 2137 3UTR 100% 13.200 9.240 N SPRTN n/a
7 TRCN0000073308 GCTACATTCACTCTTGCCTTA pLKO.1 1933 3UTR 100% 4.050 2.835 N SPRTN n/a
8 TRCN0000073311 GTACAACCACAGCTCAGAATT pLKO.1 1585 CDS 100% 0.000 0.000 N SPRTN n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006711818.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09134 pDONR223 100% 91.1% 91% None 320_321ins129;758C>T n/a
2 ccsbBroad304_09134 pLX_304 0% 91.1% 91% V5 320_321ins129;758C>T n/a
3 TRCN0000467163 TCTCTTAGGATATTAATGTATTTT pLX_317 27.1% 91.1% 91% V5 320_321ins129;758C>T n/a
4 ccsbBroadEn_12763 pDONR223 99% 40.6% 40% None (many diffs) n/a
5 ccsbBroad304_12763 pLX_304 0% 40.6% 40% V5 (many diffs) n/a
6 TRCN0000468689 AACGATGAAAGACCCATGACACAG pLX_317 55.4% 40.6% 40% V5 (many diffs) n/a
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