Construct: ORF TRCN0000467164
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001705.1_s317c1
- Derived from:
- ccsbBroadEn_05866
- DNA Barcode:
- GACTCTCCTCGTACCATCCACGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP12A (479)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467164
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 479 | ATP12A | ATPase H+/K+ transporting n... | NM_001185085.1 | 99.9% | 100% | 1605C>T |
| 2 | human | 479 | ATP12A | ATPase H+/K+ transporting n... | NM_001676.6 | 99.3% | 99.4% | 797_798insAGCATCTACTTCCCCTGT;1587C>T |
| 3 | mouse | 192113 | Atp12a | ATPase, H+/K+ transporting,... | NM_138652.2 | 84.5% | 86.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3201
- ORF length:
- 3135
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ccagaaaacc ccagaaattt actccgtgga gctcagcgga actaaggaca 121 tcgtgaaaac agacaagggg gatggcaagg agaagtatag gggtctgaag aacaactgcc 181 tggaactcaa aaagaaaaat cacaaagagg agtttcagaa agaactccat ctggatgacc 241 acaaactcag caatagggaa ttggaagaga aatatggcac agacatcatt atgggtctct 301 ccagcaccag agctgccgag ctcctggccc gggatgggcc caactccctc acccctccca 361 agcagacgcc tgagatcgtc aagttcctca agcagatggt gggggggttc tctatcctcc 421 tgtgggtggg cgcctttctc tgttggattg catatgggat tcagtactcc agcgacaagt 481 ctgcatccct gaacaacgtg tacttgggct gtgtgcttgg tctggtggtc attttaacgg 541 ggatctttgc ttattaccaa gaggcaaaaa gcaccaacat catgtccagc ttcaataaga 601 tgatccctca gcaagctctc gtcatccgag attccgagaa gaagaccatc ccttcagagc 661 agctggtggt gggggacatt gtggaggtca aaggaggaga ccagatccct gcagacatca 721 gggtgctgtc ttctcagggg tgtcgggtgg ataactcatc tctcacgggg gagtctgagc 781 cccagccccg ctcctctgag tttacccatg aaaaccccct ggaaacaaag aacatctgct 841 tctattccac aacgtgtctg gaagcatcta cttcccctgt aggcactgtc accggcatgg 901 ttatcaacac gggtgaccgc accatcattg gccatattgc ctcattggcc tcaggagttg 961 gaaatgagaa gacgcccatt gccattgaga tcgagcactt tgttcacatt gtggcaggag 1021 tggctgtctc catcggcatc cttttcttca tcatcgctgt gtccctgaag tatcaagtcc 1081 tggactccat catcttcctc attggcatca ttgtggccaa tgtgcccgag ggcctcctgg 1141 ccactgtcac tgtgaccctg tcgctgacag caaaacggat ggccaagaag aactgcctgg 1201 tgaagaacct ggaggctgtg gagaccctcg gctccacctc catcatctgc tcggacaaga 1261 ctgggacact gacccagaac aggatgacag tggcccatct gtggttcgac aatcagatct 1321 ttgtggctga caccagtgag gaccattcaa accaagtctt tgaccaaagc tctaggactt 1381 gggcctcctt atccaagata ataacattgt gtaaccgagc agagttcaag ccaggacagg 1441 aaaatgtccc catcatgaag aaagctgtga ttggagatgc ctcagaaact gctcttttaa 1501 aattctcaga ggtcattttg ggtgatgtga tggaaattag aaaaagaaac cgcaaagtag 1561 ctgaaatccc ttttaactct actaataaat ttcagctctc catccacgag atggatgacc 1621 cccacggcaa gcgcttcctc atggtgatga agggggcccc tgagcgcatt ctagagaaat 1681 gcagcaccat catgatcaac ggcgaggagc acccactgga caagagcact gccaagacct 1741 tccacacagc ctacatggag ctgggcgggt tgggcgagcg tgtgctgggt ttctgtcatc 1801 tctacctgcc agcagacgag tttccagaaa cctactcatt tgacatagac gctatgaact 1861 ttccgacctc caacctctgt tttgtgggac tcttgtcaat gatcgatccc cctcggtcca 1921 ccgtgccaga tgcagtcacc aaatgccgga gtgcagggat caaggttatt atggttactg 1981 gtgatcatcc catcacagcc aaagctattg ccaagagtgt ggggatcatt tcagccaaca 2041 gtgaaacagt ggaagacatt gcacatcgcc tcaacattgc tgtggagcaa gttaacaaac 2101 gggatgccaa ggccgctgtg gtgactggca tggagctgaa ggacatgagc tcagaacagc 2161 tggatgagat cttagccaac taccaggaga ttgtctttgc ccggacatcc ccccagcaga 2221 agctgatcat tgtggagggc tgtcagaggc aggatgctgt tgttgctgtg accggggatg 2281 gagttaatga ctctccggct ctaaagaagg cagacattgg gattgccatg gggatagcag 2341 gttctgatgc agccaaaaat gcagccgaca tggtcttgct ggacgacaac ttcgcatcca 2401 tcgtcacagg ggtggaggaa ggtcgcctga tctttgacaa cctcaagaag actattgctt 2461 attccctgac caagaacatt gccgagctgt gcccctttct gatctacatc attgtcgggc 2521 tccccctgcc cattggcacc atcaccattc tgttcattga cttggggaca gacattatcc 2581 cctccattgc cttggcgtac gagaaagctg aaagtgacat catgaacagg aagcctcgcc 2641 acaagaataa ggacaggctg gtgaaccagc cgctcgctgt gtactcatac ctgcacattg 2701 gcctcatgca agccctggga gctttccttg tgtatttcac cgtctatgca caagagggct 2761 ttctgccccg cactctcatt aaccTGCGGG TAGAATGGGA GAAGGACTAC GTGAATGACT 2821 TGAAAGACAG CTATGGGCAG GAATGGACAA GGTACCAGAG GGAATACCTA GAATGGACGG 2881 GCTACACGGC TTTCTTTGTT GGCATCCTAG TCCAGCAAAT AGCAGATCTG ATCATCAGGA 2941 AAACCCGGAG GAATTCCATC TTCCAGCAGG GTCTCTTCAG AAATAAAGTC ATCTGGGTGG 3001 GGATCACCTC ACAGATCATC ATTGGTCTGA TCCTCTCCTA TGGCCTCGGA AGTGTCACAG 3061 CCTTGAGTTT CACCATGCTT AGGGCTCAGT ACTGGTTTGT GGCTGTGCCG CACGCCATCC 3121 TGATCTGGGT GTATGATGAG GTGCGGAAGC TCTTCATCAG GCTCTACCCT GGAAGCTGGT 3181 GGGATAAGAA CATGTATTAT CACCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG 3241 CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT 3301 GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGAC TCTCCTCGTA 3361 CCATCCACGC CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat 3421 t