Construct: ORF TRCN0000467167
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016116.1_s317c1
- Derived from:
- ccsbBroadEn_13769
- DNA Barcode:
- CCGATTGATAACTCAGTGGTCTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467167
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7936 | NELFE | negative elongation factor ... | XM_006715205.3 | 88.8% | 88.8% | 578_703del |
| 2 | human | 7936 | NELFE | negative elongation factor ... | NM_002904.6 | 87.6% | 87.6% | 403_417del;593_718del |
| 3 | human | 7936 | NELFE | negative elongation factor ... | XM_017011299.2 | 74.1% | 74.1% | 0_1ins165;413_538del |
| 4 | human | 7936 | NELFE | negative elongation factor ... | XM_011514913.3 | 73.1% | 73.1% | 0_1ins165;238_252del;428_553del |
| 5 | mouse | 27632 | Nelfe | negative elongation factor ... | NM_001045863.1 | 77.1% | 81.3% | (many diffs) |
| 6 | mouse | 27632 | Nelfe | negative elongation factor ... | NM_001045864.1 | 77.1% | 81.3% | (many diffs) |
| 7 | mouse | 27632 | Nelfe | negative elongation factor ... | NM_138580.2 | 77.1% | 81.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1065
- ORF length:
- 999
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ggtgataccc cccggactga gcgaggaaga ggaggctctg cagaagaaat 121 tcaacaagct caagaaaaag aaaaaggcat tgctggctct gaagaagcaa agtagcagca 181 gcacaaccag ccaaggtggt gtcaaacgct cactatcaga gcagcctgtc atggacacag 241 ccacagcaac agagcaggca aagcagctgg tgaagtcagg agccatcagt gccatcaagg 301 ctgagaccaa gaactcaggc ttcaagcgtt ctcgaaccct tgaggggaag ttaaaggacc 361 ccgagaaggg accagtcccc actttccagc cgttccagag gagcatatct gctgatgatg 421 acctgcaaga gtcatccaga cgtccccaga ggaaatctct gtatgagagt gatcgacttc 481 gagaactagg accagatgga gaagaggcag agggcccagg ggctggtgat ggtccccctc 541 gaagctttga ctggggctat gaagaacgca gtggtgccca ctcctcagcc tcccctcccc 601 gaagccgcag ccgggaccgc agccatgaga ggaaccggga cagagaccga gagggtcctt 661 tccgcaggtc ggattcattc cctgaacggc gagcccctag gaaagggaat actctctatg 721 tatatggaga agacatgaca cccacccttc tccgtggggc cTTCTCTCCT TTTGGAAACA 781 TCATTGACCT CTCCATGGAC CCACCCAGAA ACTGTGCCTT CGTCACCTAT GAAAAGATGG 841 AGTCAGCAGA TCAGGCCGTT GCTGAGCTCA ACGGGACCCA GGTGGAGTCT GTACAGCTCA 901 AAGTCAACAT AGCCCGAAAA CAGCCCATGC TGGATGCCGC TACTGGCAAG TCTGTCTGGG 961 GCTCCCTCGC TGTCCAGAAC AGCCCTAAGG GTTGCCACCG GGACAAGAGG ACCCAGATTG 1021 TCTACAGTGA TGACGTCTAC AAGGAAAACC TTGTGGATGG CTTCTACCCA ACTTTCTTGT 1081 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1141 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1201 GAAAGGACGA CCGATTGATA ACTCAGTGGT CTTGACGCGT TAAGTCgaca atcaacctct 1261 ggattacaaa atttgtgaaa gatt