Construct: ORF TRCN0000467181
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010747.1_s317c1
- Derived from:
- ccsbBroadEn_09549
- DNA Barcode:
- CGAGCTCGCTCGCCCCCGGCTCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EGFLAM (133584)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467181
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 133584 | EGFLAM | EGF like, fibronectin type ... | NM_152403.4 | 99.9% | 99.9% | 1418C>T;3000G>A |
| 2 | human | 133584 | EGFLAM | EGF like, fibronectin type ... | NM_001205301.2 | 99.1% | 99% | 1418C>T;2465_2488del;3024G>A |
| 3 | human | 133584 | EGFLAM | EGF like, fibronectin type ... | NM_182798.3 | 76.6% | 76.3% | (many diffs) |
| 4 | human | 133584 | EGFLAM | EGF like, fibronectin type ... | NM_182801.3 | 15% | 15% | 0_1ins2571;429G>A |
| 5 | mouse | 268780 | Egflam | EGF-like, fibronectin type ... | NM_178748.6 | 84.8% | 88.3% | (many diffs) |
| 6 | mouse | 268780 | Egflam | EGF-like, fibronectin type ... | XM_017316628.1 | 84.8% | 88.3% | (many diffs) |
| 7 | mouse | 268780 | Egflam | EGF-like, fibronectin type ... | NM_001289496.1 | 84.1% | 87.5% | (many diffs) |
| 8 | mouse | 268780 | Egflam | EGF-like, fibronectin type ... | NM_001289498.1 | 72% | 73% | (many diffs) |
| 9 | mouse | 268780 | Egflam | EGF-like, fibronectin type ... | XM_017316629.1 | 70.4% | 69.5% | (many diffs) |
| 10 | mouse | 268780 | Egflam | EGF-like, fibronectin type ... | XM_006519991.1 | 65.8% | 69.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3093
- ORF length:
- 3027
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tttaatccga ggcgtcttgc tccggctcct gctcctggct tccagcctcg 121 gacccggcgc ggtgtcgctc cgagcggcca tccgaaaacc aggcaaggta gggcctcctc 181 ttgacatcaa gctgggcgca ttgaactgta cggctttcag catccagtgg aaaatgccaa 241 ggcatcctgg aagtcccatc cttgggtaca ctgtctttta ctctgaggtt ggcgcagata 301 aatccctgca ggagcagttg cacagcgtgc ctctcagccg ggacatcccg accacggagg 361 aagtgattgg agatttgaaa ccaggcactg aatatcgtgt gagcatagca gcttacagcc 421 aggctggcaa agggcggctg agctctcctc ggcatgtcac cactttgtcc caagattcct 481 gcctgcctcc tgcagctccc cagcagccac atgtcattgt ggtttcggat tctgaggtgg 541 ccctgtcttg gaaacctgga gcgagtgaag gaagcgcccc tattcagtac tattctgtgg 601 aattcatcag gccagatttc gacaagaagt ggacctcaat ccatgagcgg atccagatgg 661 actccatggt tatcaagggc ctcgatccag ataccaacta ccagtttgcc gtgagggcaa 721 tgaattccca tggccccagc ccccgcagct ggcccagtga catcatccgg accctctgcc 781 ctgaggaggc gggaagtggc cgctatggac cccgttatat caccgacatg ggagctggtg 841 aggatgatga aggatttgaa gacgacttag atttggatat ttcctttgag gaggttaaac 901 cacttcctgc taccaaagga gggaataaga aatttttggt ggaaagcaag aagatgtcta 961 tatctaaccc aaagaccatt tctaggctca tcccccctac ctcagcatct ctccctgtga 1021 ccacggtggc tccccagccc attcccatac agagaaaggg gaagaatggt gtggccataa 1081 tgtcaaggct ctttgacatg ccttgtgatg aaactctctg ctctgctgac agcttctgtg 1141 tcaatgacta cacctggggg ggctcgcgat gccagtgcac cctgggcaaa ggtggtgaga 1201 gctgctcaga agatattgtt atccagtatc ctcagttctt tggccactcc tatgtaacgt 1261 ttgaacctct gaagaattct tatcaggcat ttcaaattac tcttgaattt agggcggagg 1321 cagaggatgg cttactgctc tactgtgggg agaacgaaca cgggaggggg gatttcatgt 1381 ccctggctat catccgacgc tccctgcagt tcaggtttaa ttgtggaact ggggttgcca 1441 tcatcgtaag tgagaccaaa atcaaactag ggggttggca catggttatg ctctacagag 1501 atgggctgaa cgggctgctg cagctgaaca atggcacccc agtgacaggc cagtctcagg 1561 gccaatacag taaaattact ttccggacac ctctctatct tggtggcgct cccagcgctt 1621 actggttggt tagagcaaca gggacaaacc gaggctttca aggctgtgtg cagtcgctcg 1681 ctgtgaatgg gaggagaatt gacatgaggc cctggcccct gggaaaagca ctcagtgggg 1741 ctgatgtggg ggaatgcagc agtggaatct gtgatgaggc ctcgtgcatc catggtggca 1801 cctgcacagc aatcaaagcc gactcctaca tttgcctctg tccccttggg tttaaaggtc 1861 gacactgtga agatgctttc accttgacca ttcctcagtt cagagagtct ctgagatctt 1921 acgctgcaac tccctggcca ctggagcccc agcattacct ttccttcatg gaatttgaga 1981 tcacatttcg gccagactca ggagatggtg tcctcctgta cagctatgac acaggcagca 2041 aagacttcct gtccatcaac ttggcagggg gccacgtgga gttccgcttt gactgtggct 2101 ctgggaccgg tgtcctcagg agtgaagatc ccctcaccct gggcaactgg cacgagcttc 2161 gtgtatctcg cacagcaaag aatggaatct tacaggtgga taagcagaag atagtggagg 2221 gaatggcaga gggaggcttc acacagatta agtgcaacac agacattttc attggcggag 2281 tccccaatta tgatgatgtg aagaagaact cgggtgtcct gaagcctttc agcgggagca 2341 tccagaagat catcctgaat gaccgaacca tccatgtgaa gcatgacttc acctccggag 2401 tgaatgtgga gaatgcggcc cacccctgtg tgagagcccc ttgtgcccat gggggcagct 2461 gccggcccag gaaggagggc tatgactgtg actgcccctt gggctttgag gggcttcact 2521 gccagaaagc gatcatagaa gccattgaga tcccgcagtt tatcggccgc agttacctga 2581 cgtatgacaa cccagatatc ttgaagaggg tgtcaggatc aagatcaaat gtgttcatga 2641 ggtttaaaac aactgccaag gatggccttt tgctgtggag gggagacagc cccatgagac 2701 ccaacagcga cttcatttcc ttgggccttc gggatggagc cctcgtgtTC AGCTATAACC 2761 TGGGCAGTGG TGTGGCATCC ATCATGGTGA ATGGCTCCTT CAACGATGGT CGGTGGCACC 2821 GAGTTAAGGC CGTTAGGGAT GGCCAGTCAG GAAAGATAAC CGTGGATGAC TATGGAGCCA 2881 GAACAGGCAA ATCCCCAGGC ATGATGCGGC AGCTTAACAT CAATGGAGCT CTGTATGTGG 2941 GTGGAATGAA GGAAATTGCT CTGCACACTA ACAGGCAATA TATGAGAGGG CTCGTGGGCT 3001 GTATCTCTCA CTTCACCCTG TCCACCGATT ACCACATTTC CCTCGTGGAA GATGCCGTGG 3061 ATGGAAAAAA CATCAACACT TGTGGAGCCA AGTACCCAAC TTTCTTGTAC AAAGTGGTTG 3121 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 3181 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 3241 AGCTCGCTCG CCCCCGGCTC CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 3301 ttgtgaaaga tt