Construct: ORF TRCN0000467185
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000574.1_s317c1
- Derived from:
- ccsbBroadEn_03053
- DNA Barcode:
- CCCCCTTTTATTGTCTATAGACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM14A (28978)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467185
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28978 | TMEM14A | transmembrane protein 14A | NM_014051.4 | 100% | 100% | |
| 2 | human | 28978 | TMEM14A | transmembrane protein 14A | XM_024446409.1 | 100% | 100% | |
| 3 | mouse | 75712 | Tmem14a | transmembrane protein 14A | NM_029398.3 | 89.5% | 92.9% | (many diffs) |
| 4 | mouse | 75712 | Tmem14a | transmembrane protein 14A | XM_006495581.2 | 83.6% | 86.7% | (many diffs) |
| 5 | mouse | 75712 | Tmem14a | transmembrane protein 14A | NM_001290679.1 | 58.9% | 52.9% | (many diffs) |
| 6 | mouse | 75712 | Tmem14a | transmembrane protein 14A | XM_006495579.2 | 56.2% | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 387
- ORF length:
- 297
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGACCTGAT CGGTTTTGGT TATGCAGCCC 121 TCGTGACATT TGGAAGCATT TTTGGATATA AGCGGAGAGG TGGTGTTCCG TCTTTGATTG 181 CTGGTCTTTT TGTTGGATGT TTGGCCGGCT ATGGAGCTTA CCGTGTCTCC AATGACAAAC 241 GAGATGTAAA AGTGTCACTG TTTACAGCTT TCTTCCTGGC TACCATAATG GGTGTGAGAT 301 TTAAGAGGTC CAAGAAAATA ATGCCTGCTG GTTTGGTTGC AGGTTTAAGC CTCATGATGA 361 TCCTGAGACT TGTCTTGTTG CTGCTCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 421 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 481 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCCCCTTT 541 TATTGTCTAT AGACCCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 601 aagatt