Construct: ORF TRCN0000467204
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017911.1_s317c1
- Derived from:
- ccsbBroadEn_07334
- DNA Barcode:
- CCGGTGTTAACATGATCCTAAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WASL (8976)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467204
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8976 | WASL | WASP like actin nucleation ... | NM_003941.4 | 100% | 100% | |
| 2 | mouse | 73178 | Wasl | Wiskott-Aldrich syndrome-li... | NM_028459.2 | 90.1% | 95.4% | (many diffs) |
| 3 | mouse | 73178 | Wasl | Wiskott-Aldrich syndrome-li... | NM_001167745.1 | 31.9% | 29.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1581
- ORF length:
- 1515
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ctccgtccag cagcagccgc cgccgccgcg gagggtcacc aacgtggggt 121 ccctgttgct caccccgcag gagaacgagt ccctcttcac tttcctcggc aagaaatgtg 181 tgactatgtc ttcagcagtg gtgcagttat atgcagcaga tcggaactgt atgtggtcaa 241 agaagtgcag tggtgttgct tgtcttgtta aggacaatcc acagagatct tattttttaa 301 gaatatttga cattaaggat gggaaactat tgtgggaaca agagctatac aataactttg 361 tatataatag tcctagagga tattttcata cctttgctgg agatacttgt caagttgctc 421 ttaattttgc caatgaagaa gaagcaaaaa aatttcgaaa agcagttaca gaccttttgg 481 gccgtcgaca aaggaaatct gagaaaagac gagatccccc aaatggtcct aatctaccca 541 tggctacagt tgatataaaa aatccagaaa tcacaacaaa tagattttat ggtccacaag 601 tcaacaacat ctcccatacc aaagaaaaga agaagggaaa agctaaaaag aagagattaa 661 ccaaggcaga tataggaaca ccaagcaatt tccagcacat tggacatgtt ggttgggatc 721 caaatacagg ctttgatctg aataatttgg atccagaatt gaagaatctt ttcgatatgt 781 gtggaatctc agaggcacaa cttaaagaca gagaaacatc aaaagttata tatgacttta 841 ttgaaaaaac aggaggtgtt gaagctgtta aaaatgaact gcggaggcaa gcaccaccac 901 ctccaccacc atcaagggga gggccacctc ctcctcctcc ccctccacac aactcaggtc 961 ctcctcctcc tcctgctagg ggaagaggcg ctcctccccc accaccttca agagctccca 1021 cagctgcacc tccaccaccg cctccttcca ggccaagtgt agcagtccct ccaccaccgc 1081 caaataggat gtaccctcct ccacctccag cccttccctc ctcagcacct tcagggcctc 1141 caccaccacc tccatctgtg ttgggggtag ggccagtggc accaccccca ccgcctccac 1201 cTCCACCTCC TCCTGGGCCA CCGCCCCCGC CTGGCCTGCC TTCTGATGGG GACCATCAGG 1261 TTCCAACTAC TGCAGGAAAC AAAGCAGCTC TTTTAGATCA AATTAGAGAG GGTGCTCAGC 1321 TAAAAAAAGT GGAGCAGAAC AGTCGGCCAG TGTCCTGCTC TGGACGAGAT GCACTGTTAG 1381 ACCAGATACG ACAGGGTATC CAACTAAAAT CTGTGGCTGA TGGCCAAGAG TCTACACCAC 1441 CAACACCTGC ACCCACTTCA GGAATTGTGG GTGCATTAAT GGAAGTGATG CAGAAAAGGA 1501 GCAAAGCCAT TCATTCTTCA GATGAAGATG AAGATGAAGA TGATGAAGAA GATTTTGAGG 1561 ATGATGATGA GTGGGAAGAC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1621 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1681 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCGG TGTTAACATG 1741 ATCCTAAAGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt