Construct: ORF TRCN0000467208

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012703.1_s317c1
Derived from:
ccsbBroadEn_03888
DNA Barcode:
ACCTCCAGTCGTTTCTAGCTACTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SAV1 (60485)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467208
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 60485 SAV1 salvador family WW domain c... NM_021818.4 100% 100%
2 human 60485 SAV1 salvador family WW domain c... XM_011537057.3 91.3% 91.3% 0_1ins99
3 human 60485 SAV1 salvador family WW domain c... XM_017021568.2 91.3% 91.3% 0_1ins99
4 mouse 64010 Sav1 salvador family WW domain c... NM_022028.2 88.6% 93.5% (many diffs)
5 mouse 64010 Sav1 salvador family WW domain c... XM_030246842.1 52.2% 43.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1215
ORF length:
1149
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct gtcccgaaag aaaaccaaaa acgaagtgtc caagccggcc gaggtgcagg
121ggaagtacgt gaagaaggag acgtcgcctc tgcttcggaa tcttatgcct tcattcatcc
181ggcatggtcc aacaattcca agacgaactg atatctgtct tccagattca agccctaatg
241ccttttcaac ttctggagat gtagtttcaa gaaaccagag tttccttaga actccaattc
301aaagaacacc tcatgaaata atgagaagag aaagcaacag attatctgca ccttcttatc
361ttgccagaag tctagcagat gtccctagag agtatggttc ttctcagtca tttgtaacgg
421aagttagttt tgctgttgaa aatggagact ctggttcccg atattattat tcagacaatt
481tttttgatgg tcagagaaag cggccacttg gagatcgtgc acatgaagac tacagatatt
541atgaatacaa ccatgatctc ttccaaagaa tgccacagaa tcaggggagg catgcttcag
601gtattgggag agttgctgct acatctttag gaaatttgac taaccatggt tctgaagatt
661taccccttcc tcctggctgg tctgtggact ggacaatgag agggagaaaa tattatatag
721atcataacac aaatacaact cactggagcc atcctcttga gcgagaagga cttcctcctg
781gatgggaacg agttgagtca tccgaatttg gaacctatta tgtagatcac acaaataaga
841aggcccaata caggcatccc tgtgctccta gtgtacctcg gtatgatcaa ccacctcctg
901tcacatacca gccacagcaa actgaaagaa atcagtccct tctggtacct gcaaatccat
961atcatactgc agaaattcct gactggcttc aggtttacgc acgagcccct gtgaaatatg
1021accacattct gaagtgggaa ctcttccagc tggctgacct GGATACATAC CAGGGAATGC
1081TAAAGTTGCT CTTCATGAAA GAATTGGAGC AGATTGTTAA AATGTATGAA GCATACAGAC
1141AAGCCCTTCT TACAGAGTTG GAAAACCGAA AGCAGAGACA GCAGTGGTAT GCCCAACAAC
1201ATGGAAAAAA TTTTTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1261CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1321TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACCTCCAGTC GTTTCTAGCT
1381ACTGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)