Construct: ORF TRCN0000467208
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012703.1_s317c1
- Derived from:
- ccsbBroadEn_03888
- DNA Barcode:
- ACCTCCAGTCGTTTCTAGCTACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAV1 (60485)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467208
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60485 | SAV1 | salvador family WW domain c... | NM_021818.4 | 100% | 100% | |
| 2 | human | 60485 | SAV1 | salvador family WW domain c... | XM_011537057.3 | 91.3% | 91.3% | 0_1ins99 |
| 3 | human | 60485 | SAV1 | salvador family WW domain c... | XM_017021568.2 | 91.3% | 91.3% | 0_1ins99 |
| 4 | mouse | 64010 | Sav1 | salvador family WW domain c... | NM_022028.2 | 88.6% | 93.5% | (many diffs) |
| 5 | mouse | 64010 | Sav1 | salvador family WW domain c... | XM_030246842.1 | 52.2% | 43.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1215
- ORF length:
- 1149
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtcccgaaag aaaaccaaaa acgaagtgtc caagccggcc gaggtgcagg 121 ggaagtacgt gaagaaggag acgtcgcctc tgcttcggaa tcttatgcct tcattcatcc 181 ggcatggtcc aacaattcca agacgaactg atatctgtct tccagattca agccctaatg 241 ccttttcaac ttctggagat gtagtttcaa gaaaccagag tttccttaga actccaattc 301 aaagaacacc tcatgaaata atgagaagag aaagcaacag attatctgca ccttcttatc 361 ttgccagaag tctagcagat gtccctagag agtatggttc ttctcagtca tttgtaacgg 421 aagttagttt tgctgttgaa aatggagact ctggttcccg atattattat tcagacaatt 481 tttttgatgg tcagagaaag cggccacttg gagatcgtgc acatgaagac tacagatatt 541 atgaatacaa ccatgatctc ttccaaagaa tgccacagaa tcaggggagg catgcttcag 601 gtattgggag agttgctgct acatctttag gaaatttgac taaccatggt tctgaagatt 661 taccccttcc tcctggctgg tctgtggact ggacaatgag agggagaaaa tattatatag 721 atcataacac aaatacaact cactggagcc atcctcttga gcgagaagga cttcctcctg 781 gatgggaacg agttgagtca tccgaatttg gaacctatta tgtagatcac acaaataaga 841 aggcccaata caggcatccc tgtgctccta gtgtacctcg gtatgatcaa ccacctcctg 901 tcacatacca gccacagcaa actgaaagaa atcagtccct tctggtacct gcaaatccat 961 atcatactgc agaaattcct gactggcttc aggtttacgc acgagcccct gtgaaatatg 1021 accacattct gaagtgggaa ctcttccagc tggctgacct GGATACATAC CAGGGAATGC 1081 TAAAGTTGCT CTTCATGAAA GAATTGGAGC AGATTGTTAA AATGTATGAA GCATACAGAC 1141 AAGCCCTTCT TACAGAGTTG GAAAACCGAA AGCAGAGACA GCAGTGGTAT GCCCAACAAC 1201 ATGGAAAAAA TTTTTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1261 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1321 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACCTCCAGTC GTTTCTAGCT 1381 ACTGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt