Construct: ORF TRCN0000467222
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009509.1_s317c1
- Derived from:
- ccsbBroadEn_00171
- DNA Barcode:
- AGAGGCTATGTACACTGCCGAGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BNIP3L (665)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467222
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 665 | BNIP3L | BCL2 interacting protein 3 ... | NM_004331.3 | 100% | 100% | |
| 2 | human | 665 | BNIP3L | BCL2 interacting protein 3 ... | NM_001330491.2 | 81.7% | 81.7% | 0_1ins120 |
| 3 | mouse | 12177 | Bnip3l | BCL2/adenovirus E1B interac... | NM_009761.3 | 90.7% | 96.8% | (many diffs) |
| 4 | mouse | 12177 | Bnip3l | BCL2/adenovirus E1B interac... | XM_006518471.2 | 74.5% | 80.3% | (many diffs) |
| 5 | mouse | 12177 | Bnip3l | BCL2/adenovirus E1B interac... | XM_017315807.1 | 74.5% | 80.3% | (many diffs) |
| 6 | mouse | 100043324 | Bnip3l-ps | BCL2/adenovirus E1B interac... | XR_872527.1 | 48.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 723
- ORF length:
- 657
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gtcccaccta gtcgagccgc cgccgcccct gcacaacaac aacaacaact 121 gcgaggaaaa tgagcagtct ctgcccccgc cggccggcct caacagttcc tgggtggagc 181 tacccatgaa cagcagcaat ggcaatgata atggcaatgg gaaaaatggg gggctggaac 241 acgtaccatc ctcatcctcc atccacaatg gagacatgga gaagattctt ttggatgcac 301 aacatgaatc aggacagagt agttccagag gcagttctca ctgtgacagc ccTTCGCCAC 361 AAGAAGATGG GCAGATCATG TTTGATGTGG AAATGCACAC CAGCAGGGAC CATAGCTCTC 421 AGTCAGAAGA AGAAGTTGTA GAAGGAGAGA AGGAAGTCGA GGCTTTGAAG AAAAGTGCGG 481 ACTGGGTATC AGACTGGTCC AGTAGACCCG AAAACATTCC ACCCAAGGAG TTCCACTTCA 541 GACACCCTAA ACGTTCTGTG TCTTTAAGCA TGAGGAAAAG TGGAGCCATG AAGAAAGGGG 601 GTATTTTCTC CGCAGAATTT CTGAAGGTGT TCATTCCATC TCTCTTCCTT TCTCATGTTT 661 TGGCTTTGGG GCTAGGCATC TATATTGGAA AGCGACTGAG CACACCCTCT GCCAGCACCT 721 ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 781 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 841 GGCTTTATAT ATCTTGTGGA AAGGACGAAG AGGCTATGTA CACTGCCGAG AGACGCGTTA 901 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt