Construct: ORF TRCN0000467238
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013060.1_s317c1
- Derived from:
- ccsbBroadEn_02558
- DNA Barcode:
- GGAACCGAGTTCATGCCAATAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAGED2 (10916)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467238
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10916 | MAGED2 | MAGE family member D2 | NM_014599.6 | 100% | 100% | |
| 2 | human | 10916 | MAGED2 | MAGE family member D2 | NM_177433.3 | 100% | 100% | |
| 3 | human | 10916 | MAGED2 | MAGE family member D2 | NM_201222.3 | 100% | 100% | |
| 4 | mouse | 80884 | Maged2 | melanoma antigen, family D, 2 | NM_001199246.1 | 87.2% | 86.2% | (many diffs) |
| 5 | mouse | 80884 | Maged2 | melanoma antigen, family D, 2 | NM_030700.2 | 87.2% | 86.2% | (many diffs) |
| 6 | mouse | 80884 | Maged2 | melanoma antigen, family D, 2 | XM_006528997.1 | 87.2% | 86.2% | (many diffs) |
| 7 | mouse | 80884 | Maged2 | melanoma antigen, family D, 2 | XM_006528998.3 | 87.2% | 86.2% | (many diffs) |
| 8 | mouse | 80884 | Maged2 | melanoma antigen, family D, 2 | XM_006528999.3 | 87.2% | 86.2% | (many diffs) |
| 9 | mouse | 80884 | Maged2 | melanoma antigen, family D, 2 | XM_011247882.1 | 87.1% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1884
- ORF length:
- 1818
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgacacaagc gagagtggtg caggtctaac tcgcttccag gctgaagctt 121 cagaaaagga cagtagctcg atgatgcaga ctctgttgac agtgacccag aatgtggagg 181 tcccagagac accgaaggcc tcaaaggcac tggaggtctc agaggatgtg aaggtctcaa 241 aagcctctgg ggtctcaaag gccacagagg tctcaaagac cccagaggct cgggaggcac 301 ctgccaccca ggcctcatct actactcagc tgactgatac ccaggttctg gcagctgaaa 361 acaagagtct agcagctgac accaagaaac agaatgctga cccgcaggct gtgacaatgc 421 ctgccactga gaccaaaaag gtcagccatg tggctgatac aaaggtcaat acaaaggctc 481 aggagactga ggctgcaccc tctcaggccc cagcagatga acctgagcct gagagtgcag 541 ctgcccagtc tcaggagaat caggatactc ggcccaaggt caaagccaag aaagcccgaa 601 aggtgaagca tctggatggg gaagaggatg gcagcagtga tcagagtcag gcttctggaa 661 ccacaggtgg ccgaagggtc tcaaaggccc taatggcctc aatggcccgc agggcttcaa 721 ggggtcccat agccttttgg gcccgcaggg catcaaggac tcggttggct gcttgggccc 781 ggagagcctt gctctccctg agatcaccta aagcccgtag gggcaaggct cgccgtagag 841 ctgccaagct ccagtcatcc caagagcctg aagcaccacc acctcgggat gtggcccttt 901 tgcaagggag ggcaaatgat ttggtgaagt accttttggc taaagaccag acgaagattc 961 ccatcaagcg ctcggacatg ctgaaggaca tcatcaaaga atacactgat gtgtaccccg 1021 aaatcattga acgagcaggc tattccttgg agaaggtatt tgggattcaa ttgaaggaaa 1081 ttgataagaa tgaccacttg tacattcttc tcagcacctt agagcccact gatgcaggca 1141 tactgggaac gactaaggac tcacccaagc tgggtctgct catggtgctt cttagcatca 1201 tcttcatgaa tggaaatcgg tccagtgagg ctgtcatctg ggaggtgctg cgcaagttgg 1261 ggctgcgccc tgggatacat cattcactct ttggggacgt gaagaagctc atcactgatg 1321 agtttgtgaa gcagaagtac ctggactatg ccagagtccc caatagcaat ccccctgaat 1381 atgagttctt ctggggcctg cgctcttact atgagaccag caagatgaaa gtcctcaagt 1441 ttgcctgcaa ggtacaaaag aaggatccca aggaatgggc agctcagtac cgagaggcga 1501 tggaagcgga tttgaaggct gcagctgagg ctgcagctga agccaaggct agggccgaga 1561 ttAGAGCTCG AATGGGCATT GGGCTCGGCT CGGAGAATGC TGCCGGGCCC TGCAACTGGG 1621 ACGAAGCTGA TATCGGACCC TGGGCCAAAG CCCGGATCCA GGCGGGAGCA GAAGCTAAAG 1681 CCAAAGCCCA AGAGAGTGGC AGTGCCAGCA CTGGTGCCAG TACCAGTACC AATAACAGTG 1741 CCAGTGCCAG TGCCAGCACC AGTGGTGGCT TCAGTGCTGG TGCCAGCCTG ACCGCCACTC 1801 TCACATTTGG GCTCTTCGCT GGCCTTGGTG GAGCTGGTGC CAGCACCAGT GGCAGCTCTG 1861 GTGCCTGTGG TTTCTCCTAC AAGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1921 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1981 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG GAACCGAGTT 2041 CATGCCAATA GCCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2101 att