Construct: ORF TRCN0000467239
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013509.1_s317c1
- Derived from:
- ccsbBroadEn_06836
- DNA Barcode:
- ATTCAGTTTTGCAGACGGGCGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAD23B (5887)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467239
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5887 | RAD23B | RAD23 homolog B, nucleotide... | NM_002874.5 | 99.8% | 99.7% | 12C>T;746C>T |
| 2 | human | 5887 | RAD23B | RAD23 homolog B, nucleotide... | NM_001244713.1 | 94.7% | 94.3% | 0_1ins63;2T>C;683C>T |
| 3 | human | 5887 | RAD23B | RAD23 homolog B, nucleotide... | NM_001244724.1 | 82.3% | 82.1% | 0_1ins216;530C>T |
| 4 | mouse | 19359 | Rad23b | RAD23 homolog B, nucleotide... | NM_009011.4 | 86.6% | 89.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1293
- ORF length:
- 1227
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggtcactctg aagaccctcc agcagcagac cttcaagata gacattgacc 121 ccgaggagac ggtgaaagca ctgaaagaga agattgaatc tgaaaagggg aaagatgcct 181 ttccagtagc aggtcaaaaa ttaatttatg caggcaaaat cctcaatgat gatactgctc 241 tcaaagaata taaaattgat gagaaaaact ttgtggtggt tatggtgacc aaacccaaag 301 cagtgtccac accagcacca gctacaactc agcagtcagc tcctgccagc actacagcag 361 ttacttcctc caccaccaca actgtggctc aggctccaac ccctgtccct gccttggccc 421 ccacttccac acctgcatcc atcactccag catcagcgac agcatcttct gaacctgcac 481 ctgctagtgc agctaaacaa gagaagcctg cagaaaagcc agcagagaca ccagtggcta 541 ctagcccaac agcaactgac agtacatcgg gtgattcttc tcggtcaaac ctttttgaag 601 atgcaacgag tgcacttgtg acgggtcagt cttacgagaa tatggtaact gagatcatgt 661 caatgggcta tgaacgagag caagtaattg cagccctgag agccagtttc aacaaccctg 721 acagagcagt ggagtatctt ttaatgggaa tccctggaga tagagaaagt caggctgtgg 781 ttgacccccc tcaagcagct agtactgggg ttcctcagtc ttcagcagtg gctgcagctg 841 cagcaactac gacagcaaca actacaacaa caagttctgg aggacatccc cttgaatttt 901 tacggaatca gcctcagttt caacagatga gacaaattat tcagcagaat ccttccttgc 961 ttccagcgtt actacagcag ataggtcgag agaaTCCTCA ATTACTTCAG CAAATTAGCC 1021 AACACCAGGA GCATTTTATT CAGATGTTAA ATGAACCAGT TCAAGAAGCT GGTGGTCAAG 1081 GAGGAGGAGG TGGAGGTGGC AGTGGAGGAA TTGCAGAAGC TGGAAGTGGT CATATGAACT 1141 ACATTCAAGT AACACCTCAG GAAAAAGAAG CTATAGAAAG GTTAAAGGCA TTAGGATTTC 1201 CTGAAGGACT TGTGATACAA GCGTATTTTG CTTGTGAGAA GAATGAGAAT TTGGCTGCCA 1261 ATTTTCTTCT ACAGCAGAAC TTTGATGAAG ATTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1321 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1381 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 1441 TCAGTTTTGC AGACGGGCGG CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1501 ttgtgaaaga tt