Construct: ORF TRCN0000467277
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012770.1_s317c1
- Derived from:
- ccsbBroadEn_07892
- DNA Barcode:
- AGGTCTACGGTTGTTGCAACCCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRRC8B (23507)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467277
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23507 | LRRC8B | leucine rich repeat contain... | NM_001134476.2 | 99.9% | 100% | 21A>G;39C>T |
| 2 | human | 23507 | LRRC8B | leucine rich repeat contain... | NM_001369817.1 | 99.9% | 100% | 21A>G;39C>T |
| 3 | human | 23507 | LRRC8B | leucine rich repeat contain... | NM_001369819.1 | 99.9% | 100% | 21A>G;39C>T |
| 4 | human | 23507 | LRRC8B | leucine rich repeat contain... | NM_015350.4 | 99.9% | 100% | 21A>G;39C>T |
| 5 | human | 23507 | LRRC8B | leucine rich repeat contain... | XM_005270703.5 | 99.9% | 100% | 21A>G;39C>T |
| 6 | human | 23507 | LRRC8B | leucine rich repeat contain... | XM_011541143.2 | 99.9% | 100% | 21A>G;39C>T |
| 7 | human | 23507 | LRRC8B | leucine rich repeat contain... | XM_011541144.2 | 99.9% | 100% | 21A>G;39C>T |
| 8 | human | 23507 | LRRC8B | leucine rich repeat contain... | XM_011541146.3 | 99.9% | 100% | 21A>G;39C>T |
| 9 | human | 23507 | LRRC8B | leucine rich repeat contain... | XM_017000883.1 | 99.9% | 100% | 21A>G;39C>T |
| 10 | human | 23507 | LRRC8B | leucine rich repeat contain... | XM_017000884.1 | 99.9% | 100% | 21A>G;39C>T |
| 11 | human | 23507 | LRRC8B | leucine rich repeat contain... | XM_011541148.2 | 89.9% | 88.7% | (many diffs) |
| 12 | human | 23507 | LRRC8B | leucine rich repeat contain... | XM_017000886.1 | 89.9% | 88.7% | (many diffs) |
| 13 | mouse | 433926 | Lrrc8b | leucine rich repeat contain... | NM_001033550.2 | 83.9% | 93.6% | (many diffs) |
| 14 | mouse | 433926 | Lrrc8b | leucine rich repeat contain... | XM_011249508.2 | 83.9% | 93.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2475
- ORF length:
- 2409
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaaa 61 ttggcatgat tacactaact gagctgaaat gcttagcaga tgctcagtca tcttatcaca 121 tcttaaaacc atggtgggac gtcttctggt attacatcac actgatcatg ctgctggtgg 181 ccgtgctggc cggagctctc cagctgacgc agagcagggt tctgtgctgt cttccatgca 241 aagtggaatt tgacaatcac tgtgccgtgc cttgggacat cctgaaagcc agcatgaaca 301 catcctctaa tcctgggaca ccgcttccgc tccccctccg aattcagaat gacctccacc 361 gacagcagta ctcctatatt gatgccgtct gttacgagaa acagctccat tggtttgcaa 421 agtttttccc ctatctggtg ctcttgcaca cgctcatctt tgcagcctgc agcaactttt 481 ggcttcacta ccccagtacc agttccaggc tcgagcattt tgtggccatc cttcacaagt 541 gcttcgattc tccatggacc acccgcgccc tttcagaaac agtggctgag cagtcagtga 601 ggcctctgaa actctccaag tccaagattt tgctttcgtc ctcagggtgt tcagctgaca 661 tagattccgg caaacagtca ttgccctacc cacagccagg tttggagtca gctggcatag 721 aaagcccaac ttccagtgtc ctggacaaga aggagggtga acaggccaaa gccatctttg 781 aaaaagtgaa aagattccgc atgcatgtgg agcagaagga catcatttat agagtatatc 841 tgaaacagat aatagtcaaa gtcattttgt ttgtgctcat cataacttat gttccatatt 901 ttttaaccca catcactctt gaaatcgact gttcagttga tgtgcaggct tttacaggat 961 ataagcgcta ccagtgtgtc tattccttgg cagaaatctt taaggtcctg gcttcatttt 1021 atgtcatttt ggttatactt tatggtctga cctcttccta cagcctgtgg tggatgctga 1081 ggagttccct gaagcaatat tcctttgagg cgttaagaga aaaaagcaac tacagtgaca 1141 tccctgatgt caagaatgac tttgccttca tccttcatct ggctgatcag tatgatcctc 1201 tttattccaa acgcttctcc atattcctat cagaggtcag tgagaacaaa ctgaaacaga 1261 tcaacctcaa taatgaatgg acagttgaga aactgaaaag taagcttgtg aaaaatgccc 1321 aggacaagat agaactgcat ctttttatgc tcaacggtct tccagacaat gtctttgagt 1381 taactgaaat ggaagtgcta agcctggagc ttatcccaga ggtgaagctg ccctctgcag 1441 tctcacagct ggtcaacctc aaggagcttc gtgtgtacca ttcatctctg gtcgtagacc 1501 atcctgcact ggcctttcta gaggagaatt taaaaatcct ccgcctgaaa tttactgaaa 1561 tgggaaaaat cccacgctgg gtatttcacc tcaagaatct caaggaactt tatctttcgg 1621 gctgtgttct ccctgaacag ttgagtacta tgcagttgga gggctttcag gacttaaaaa 1681 atctaaggac cctgtacttg aagagcagcc tctcccggat cccacaagtt gttacagacc 1741 tcctgccttc attgcagaaa ctgtcccttg ataatgaggg aagcaaactg gttgtgttga 1801 acaacttgaa aaagatggtc aatctgaaaa gcctagaact gatcagctgt gacctggaac 1861 gcatcccaca ttccattttc agcctgaata atttgcatga gttagaccta agggaaaata 1921 accttaaaac tgtggaagag atcattagct ttcagcatct tcagaatctt tcctgcttaa 1981 agttgtggca caataacatt gcttatattc ctgcacagat tggggcatta tctaacctag 2041 agcagctctc tttggaccat aataatattg agaatctgcc cttgcagctt ttcctatgca 2101 ctaaactaca ttatttGGAT CTAAGCTATA ACCACTTGAC CTTCATTCCA GAAGAAATCC 2161 AGTATCTGAG TAATTTGCAG TACTTTGCTG TGACCAACAA CAATATTGAG ATGCTACCAG 2221 ATGGGCTGTT TCAGTGCAAA AAGCTGCAGT GTTTACTTTT GGGGAAAAAT AGCTTGATGA 2281 ATTTGTCCCC TCATGTGGGT GAGCTGTCAA ACCTTACTCA TCTGGAGCTC ATTGGTAATT 2341 ACCTGGAAAC ACTTCCTCCT GAACTAGAAG GATGTCAGTC CCTAAAACGG AACTGTCTGA 2401 TTGTTGAGGA GAACTTGCTC AATACTCTTC CTCTCCCTGT AACAGAACGT TTACAGACGT 2461 GCTTAGACAA ATGTTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2521 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2581 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGGTCTACGG TTGTTGCAAC 2641 CCATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt