Construct: ORF TRCN0000467289
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014014.1_s317c1
- Derived from:
- ccsbBroadEn_11185
- DNA Barcode:
- TTGGGCCAAGATATCTAAGAACAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TEAD1 (7003)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467289
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7003 | TEAD1 | TEA domain transcription fa... | NM_021961.6 | 82% | 82% | 1_45del;329_330insGGTAACAAGCAT;699_872del |
| 2 | mouse | 21676 | Tead1 | TEA domain family member 1 | NM_009346.3 | 78.7% | 85.3% | (many diffs) |
| 3 | mouse | 21676 | Tead1 | TEA domain family member 1 | NM_001166585.1 | 77.8% | 84.3% | (many diffs) |
| 4 | mouse | 21676 | Tead1 | TEA domain family member 1 | XM_006507563.2 | 77.8% | 84.3% | (many diffs) |
| 5 | mouse | 21676 | Tead1 | TEA domain family member 1 | XM_006507562.2 | 77.2% | 83.3% | (many diffs) |
| 6 | mouse | 21676 | Tead1 | TEA domain family member 1 | XM_011241723.1 | 76.4% | 82.4% | (many diffs) |
| 7 | mouse | 21676 | Tead1 | TEA domain family member 1 | NM_001166584.1 | 75% | 81.1% | (many diffs) |
| 8 | mouse | 21676 | Tead1 | TEA domain family member 1 | XM_006507561.2 | 74% | 80.2% | (many diffs) |
| 9 | mouse | 21676 | Tead1 | TEA domain family member 1 | XM_006507564.2 | 73.8% | 80.2% | (many diffs) |
| 10 | mouse | 21676 | Tead1 | TEA domain family member 1 | XM_006507565.2 | 72.8% | 79.2% | (many diffs) |
| 11 | mouse | 21676 | Tead1 | TEA domain family member 1 | XM_011241724.1 | 63.6% | 66.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1140
- ORF length:
- 1071
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaaggatg agtgactctg cagataagcc aattgacaat gatgcagaag 121 gggtctggag ccccgacatc gagcaaagct ttcaggaggc cctggctatc tatccaccat 181 gtgggaggag gaaaatcatc ttatcagacg aaggcaaaat gtatggtagg aatgaattga 241 tagccagata catcaaactc aggacaggca agacgaggac cagaaaacag gtgtctagtc 301 acattcaggt tcttgccaga aggaaatctc gtgattttca ttccaagcta aaggtaacaa 361 gcatggatca gactgcaaag gataaggccc tgcagcacat ggcggccatg tcctcagccc 421 agatcgtctc ggccactgcc attcataaca agctggggct gcctgggatt ccacgcccga 481 ccttcccagg ggcgccgggg ttctggccgg gaatgattca aacagggcag ccaggatcct 541 cacaagacgt caagcctttt gtgcagcagg cctaccccat ccagccagcg gtcacagccc 601 ccattccagg gtttgagcct gcatcggccc cagctccctc agtccctgcc tggcaaggtc 661 gctccattgg cacaaccaag cttcgcctgg tggaattttc agcttttctc gagcagcagc 721 gagacccaga ctcggctgat ttaaactgca atattcaaga tgatgctggg gctttttatg 781 gtgtaaccag tcagtacgag agttcTGAAA ATATGACAGT CACCTGTTCC ACCAAAGTTT 841 GCTCCTTTGG GAAGCAAGTA GTAGAAAAAG TAGAGACGGA GTATGCAAGG TTTGAGAATG 901 GCCGATTTGT ATACCGAATA AACCGCTCCC CAATGTGTGA ATATATGATC AACTTCATCC 961 ACAAGCTCAA ACACTTACCA GAGAAATATA TGATGAACAG TGTTTTGGAA AACTTCACAA 1021 TTTTATTGGT GGTAACAAAC AGGGATACAC AAGAAACTCT ACTCTGCATG GCCTGTGTGT 1081 TTGAAGTTTC AAATAGTGAA CACGGAGCAC AACATCATAT TTACAGGCTT GTAAAGGACT 1141 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1201 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1261 TTTATATATC TTGTGGAAAG GACGATTGGG CCAAGATATC TAAGAACAGA CGCGTTAAGT 1321 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt