Construct: ORF TRCN0000467293
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005363.1_s317c1
- Derived from:
- ccsbBroadEn_01886
- DNA Barcode:
- GAACAAGGCTCTTGCCCGTGGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNN (8303)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467293
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8303 | SNN | stannin | NM_003498.6 | 100% | 100% | |
| 2 | human | 8303 | SNN | stannin | XM_017023742.1 | 100% | 100% | |
| 3 | human | 8303 | SNN | stannin | XM_017023741.1 | 65.6% | 65.6% | 1_138del |
| 4 | mouse | 20621 | Snn | stannin | NM_009223.3 | 89% | 97.7% | (many diffs) |
| 5 | mouse | 20621 | Snn | stannin | XM_006521880.1 | 89% | 97.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 354
- ORF length:
- 264
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCTATTAT GGACCACAGC CCCACCACGG 121 GCGTGGTCAC AGTCATCGTC ATCCTCATTG CCATCGCGGC CCTGGGGGCC TTGATCCTGG 181 GCTGCTGGTG CTACCTGCGG CTGCAGCGCA TCAGCCAGTC AGAGGACGAG GAGAGCATCG 241 TGGGGGATGG GGAGACCAAG GAACCCTTCC TGCTGGTGCA GTATTCGGCC AAGGGACCGT 301 GCGTGGAGAG AAAGGCCAAG CTGATGACTC CCAACGGCCC GGAAGTCCAC GGCTGCCCAA 361 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 421 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 481 TATCTTGTGG AAAGGACGAG AACAAGGCTC TTGCCCGTGG TATACGCGTT AAGTCgacaa 541 tcaacctctg gattacaaaa tttgtgaaag att