Construct: ORF TRCN0000467300
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000635.1_s317c1
- Derived from:
- ccsbBroadEn_07237
- DNA Barcode:
- ATTTACCCCAGACATTCGTTGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BBOX1 (8424)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467300
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8424 | BBOX1 | gamma-butyrobetaine hydroxy... | NM_003986.3 | 99.8% | 100% | 408C>T;906G>A |
| 2 | human | 8424 | BBOX1 | gamma-butyrobetaine hydroxy... | XM_005253159.2 | 99.8% | 100% | 408C>T;906G>A |
| 3 | human | 8424 | BBOX1 | gamma-butyrobetaine hydroxy... | XM_005253160.2 | 99.8% | 100% | 408C>T;906G>A |
| 4 | human | 8424 | BBOX1 | gamma-butyrobetaine hydroxy... | XM_005253161.2 | 99.8% | 100% | 408C>T;906G>A |
| 5 | human | 8424 | BBOX1 | gamma-butyrobetaine hydroxy... | XM_011520402.1 | 99.8% | 100% | 408C>T;906G>A |
| 6 | human | 8424 | BBOX1 | gamma-butyrobetaine hydroxy... | XM_017018403.2 | 99.8% | 100% | 408C>T;906G>A |
| 7 | mouse | 170442 | Bbox1 | butyrobetaine (gamma), 2-ox... | NM_130452.1 | 86.4% | 89.1% | (many diffs) |
| 8 | mouse | 170442 | Bbox1 | butyrobetaine (gamma), 2-ox... | XM_006498822.3 | 86.4% | 89.1% | (many diffs) |
| 9 | mouse | 170442 | Bbox1 | butyrobetaine (gamma), 2-ox... | XM_006498823.3 | 86.4% | 89.1% | (many diffs) |
| 10 | mouse | 170442 | Bbox1 | butyrobetaine (gamma), 2-ox... | XR_001781096.1 | 35.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1227
- ORF length:
- 1161
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttgtaccatc caaaaggcag aagcacttga cggggctcat ttgatgcaga 121 tcctctggta tgatgaggaa gagtctctct acccagctgt atggttgaga gacaactgtc 181 cgtgctctga ttgctacctg gattctgcaa aagcacggaa acttctagtg gaagctcttg 241 atgtgaacat tggaattaaa ggcttgatat ttgacagaaa aaaggtgtac atcacatggc 301 ccgatgagca ttacagtgaa ttccaggctg attggctgaa gaaaagatgc ttttccaagc 361 aggccagagc aaagctccaa agagaattgt tttttccaga atgccaatac tggggctcag 421 agctccagct acccactttg gattttgaag atgttttaag atatgatgaa catgcataca 481 agtggctctc caccctcaag aaagtaggca tagtaagact caccggagca tctgacaaac 541 caggagaagt ttcaaaactt gggaaaagga tgggtttcct ctatctcaca ttttatggac 601 atacttggca agtgcaagac aaaatcgatg caaacaatgt ggcttacaca actgggaagc 661 taagctttca cactgattat ccagccctcc atcatccacc tggggttcag cttcttcact 721 gcataaagca aacagtcaca gggggtgatt cagaaattgt agatgggttt aatgtgtgcc 781 aaaaactaaa gaaaaataat ccTCAGGCAT TCCAGATTTT GTCCTCTACC TTTGTGGACT 841 TTACAGACAT TGGAGTGGAT TACTGTGATT TTTCTGTACA ATCAAAACAT AAAATTATAG 901 AGTTAGATGA TAAAGGCCAA GTGGTTCGCA TCAACTTCAA TAACGCAACT AGGGACACAA 961 TATTTGATGT ACCTGTTGAA AGAGTTCAGC CTTTTTATGC TGCTCTGAAG GAGTTTGTTG 1021 ACCTCATGAA CAGCAAAGAA TCCAAGTTTA CCTTCAAGAT GAATCCAGGT GATGTGATTA 1081 CTTTTGATAA CTGGCGCTTA CTTCATGGCC GACGTAGCTA TGAAGCAGGA ACTGAGATAT 1141 CCCGCCATCT AGAAGGAGCT TATGCTGACT GGGATGTGGT CATGTCAAGG CTTCGTATCT 1201 TAAGGCAGAG GGTGGAGAAT GGAAACTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1261 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1321 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATTTACCC 1381 CAGACATTCG TTGTGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1441 aagatt